Pengaruh Desinfektan dan Lama Perendaman pada Sterilisasi Eksplan Daun Sukun (Artocarpus altilis (Parkinson ex. F.A Zorn) Fosberg)

NUR ASNI SETIANI, - and FITRI NURWINDA, - and DEWI ASTRIANY, - (2018) Pengaruh Desinfektan dan Lama Perendaman pada Sterilisasi Eksplan Daun Sukun (Artocarpus altilis (Parkinson ex. F.A Zorn) Fosberg). Biotropika: Journal of Tropical Biology, 6 (3). pp. 78-82. ISSN p-ISSN: 2302-7282, e-ISSN: 2549-8703)

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Abstract

Breadfruit leaves (Artocarpusaltilis) is a herb that is widely used forherbal medicine. Tissue culture becomes an alternative method to enhancethe productivity ofbreadfruit leavesand secondary metabolitesthat it produces. The success of plant propagationthrough tissue culture is determined by sterilization technique. This study aimedtoobtain sterilization method by determiningthe effect of 5.25% sodium hypochlorite and 70% alcohol and soaking time on the decrease of contamination. In this study there were 10 groups consisting of positive control, negative control, immersion with 70% alcohol for 5 and 10 minutes, immersion with 5.25% sodium hypochlorite for 5 and 10 minutes, and combination of 5.25 % sodium hypochlorite and 70% alcohol for 5 minutes and 10 minutes. Parametersobserved werefirst time of contamination, percentage of fungal and bacterial contamination, and appearance of explants observed for 40 days. The results of the use of each disinfectant, 70% alcohol and 5.25% sodium hypochlorite with 10 minutes immersion time showed no presence fungal and bacterial contamination, but in the treatment of sodium hypochlorite 5.25%, the explants become brownish. The combination of both disinfectants indicatedthe presence of fungal and bacterial contamination and thechanges of explants color.The best sterilization technique of breadfruit leavescan be done by immersion using 70% alcohol for 10 minutes.

Item Type:	Article
Uncontrolled Keywords:	alcohol, breadfruit leaves (Artocarpus altilis), explants, sodium hypochlorite, sterilization
Subjects:	Q Science > Q Science (General)
Divisions:	Lembaga Penelitian dan Pengabdian Masyarakat > Publikasi Penelitian
Depositing User:	- Dewi Astriany -
Date Deposited:	20 Aug 2024 06:41
Last Modified:	20 Aug 2024 06:41
URI:	http://repository.stfi.ac.id/id/eprint/985

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