DEWI ASTRIANY, - and UMI BAROROH, - and TAUFIK RAMDANI TOHARI, - and SYARIF HAMDANI, - (2023) Expression and Characterization of Single-Chain Fragment Variable Antibody in Escherichia coli for Detection of Dengue Virus NS1 Antigen. In: International Conference on Contemporary Science and Clinical Pharmacy (ICCSCP), Universitas Andalas, 30-31 Oktober 2023, Fakultas Farmasi Universitas Andalas.
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Expression and Characterization of Single-Chain Fragment Variable Antibody in Escherichia coli for Detection of Dengue Virus NS1 Antigen_ICCSCP 2023 compressed.pdf Download (1MB) |
Abstract
Dengue fever is still a major health concern in tropical and subtropical regions around the world. To give improved treatment, early detection and effective therapy should be created. To confirm dengue infection, single-chain Fragment variable (scFv) antibodies can be utilised as a component of the diagnostic technique. There have been reports of scFv activity with high affinity to the NS1 dengue virus antigen. In the previous study, we constructed and expressed a vector carrying the anti-NS1 scFv gene. The recombinant proteins were successfully produced in the E. coli BL21(DE3) system in the Luria Bertani medium using a 0.5 mM final concentration of IPTG induction. The result showed that scFv found as a approximately 35 kDa protein characterised by SDS-PAGE method. It can be concluded that the soluble scFv anti-NS1 obtained can be a suitable candidate for the development of a diagnostic kit component for dengue disease.
| Item Type: | Conference or Workshop Item (Speech) |
|---|---|
| Uncontrolled Keywords: | scFv, NS1 antigen, dengue virus, expression, E. coli |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Lembaga Penelitian dan Pengabdian Masyarakat > Publikasi Penelitian |
| Depositing User: | - Dewi Astriany - |
| Date Deposited: | 22 Aug 2024 08:35 |
| Last Modified: | 22 Aug 2024 08:35 |
| URI: | http://repository.stfi.ac.id/id/eprint/976 |
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