Correlation of Total Phenolic, Flavonoid and Carotenoid Content of Phyllanthus emblica Extract from Bandung with DPPH Scavenging Activities

SANI NURLAELA FITRIANSYAH, - and DIAH LIA AULIFA, - and YESSI FEBRIANI, - and EMI SAPITRI, - (2018) Correlation of Total Phenolic, Flavonoid and Carotenoid Content of Phyllanthus emblica Extract from Bandung with DPPH Scavenging Activities. Pharmacognosy Journal, 10 (3). pp. 447-452. ISSN 0975-3575

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Abstract

Introduction: Many potential compounds have antioxidant activity, such as the flavonoid group, phenolics and carotenoids. Phyllanthus emblica is widespread in Bandung-Indonesia and is a very potent as an antioxidant activity. Antioxidant activity and correlation with total flavonoids, phenolics and carotenoids from Phyllantus extract from Bandung-Indonesia have not been reported. The aim of this research were to determine the antioxidant activity from extract of various parts of P. emblica and its correlation of antioxidant activity with the total flavonoid, phenolics and carotenoid. Method: Successive extractions of various part of P.emblica were performed by maceration using differrent polarity solvent n-hexane, ethyl acetate and ethanol. The antioxidant activity of each extracts was performed using DPPH (2.2-Diphenyl-1-Picrylhydrazil) method. The determination of total flavonoids, phenolics and carotenoids were performed by UV-Spectrophotometry. Antioxidant activity was demonstrated by IC50 and its correlation to total flavonoids, phenolics and carotenoids using the Pearson’s method. Result: The highest antioxidant activity was given by fruit ethyl acetate (BE) extract with IC50 3.032 μg/mL. Etyl acetate extract of stem bark P.emblica (KE) had the highest of total phenol content (12.818 g GAE/100 g), ethanol extract of leaves P.emblica (DO) had the highest of total flavonoid content (3.594 g QE/100 g), and n-hexane extract of leave (DN) had the highest of total carotenoid content (0.759 g BE/100 g). Conclusion: According to coeficient correlation Pearson’s between P. emblica extract with IC50 of DPPH scavengging activities, suggested that flavonoid and phenolic compound in stem bark extract and leaves extract of P. emblica were contributor major in its antioxidant activity with DPPH methode, and its same with carotenoid content in leaves extract of P. emblica.

Item Type:	Article
Uncontrolled Keywords:	Antioxidants, Crotenoids, Flavonoids, Phenols, Phyllantus emblica
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Lembaga Penelitian dan Pengabdian Masyarakat > Publikasi Penelitian
Depositing User:	- Sani Nurlaela Fitriansyah -
Date Deposited:	26 Jul 2024 04:05
Last Modified:	09 Aug 2024 03:50
URI:	http://repository.stfi.ac.id/id/eprint/736

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