SYIFA NAILA, - (2026) PENETAPAN KADAR FENOLIK TOTAL, FLAVONOID TOTAL DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK KACANG GUDE (Cajanus cajan (L.) Millsp.). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Kacang gude (Cajanus cajan (L.) Millsp.) memiliki kandungan alkaloid, fenolik, flavonoid, saponin tanin, dan terpenoid yang diketahui memiliki potensi sebagai aktivitas antioksidan yang dapat berperan dalam pencegahan penyakit degeneratif akibat radikal bebas. Penelitian mengenai kandungan senyawa aktif kacang gude di Indonesia masih terbatas, sehingga diperlukan kajian awal untuk mengeksplorasi potensinya sebagai bahan pangan berklaim berbasis sumber daya lokal. Penelitian ini bertujuan menentukan kadar fenolik dan flavonoid total, aktivitas antioksidan, serta mengidentifikasi senyawa isoflavon genistein dan daidzein pada ekstrak metanol 70% kacang gude. Ekstraksi dilakukan menggunakan metode Ultrasound Assisted Extraction (UAE). Kadar fenolik total ditentukan dengan metode FolinCiocalteu, kadar flavonoid total dengan metode Chang menggunakan AlCl₃, aktivitas antioksidan diuji dengan metode peredaman radikal DPPH. Hasil penelitian menunjukkan kadar fenolik total ekstrak sebesar 6,772 mg GAE/g dan kadar flavonoid total sebesar 2,182 mg QE/g ekstrak. Uji aktivitas antioksidan menghasilkan nilai IC50 sebesar 593,23 ppm, yang tergolong tidak aktif jika dibandingkan dengan asam askorbat sebagai kontrol positif (IC50 6,29 ppm). Penelitian ini memberikan informasi ilmiah awal mengenai potensi kacang gude sebagai sumber senyawa fenolik dan flavonoid yang dapat dikembangkan sebagai bahan pangan berklaim berbasis sumber daya lokal bagi kesehatan. ------ Pigeon pea (Cajanus cajan (L.) Millsp.), locally known as "kacang gude," is a native legume known to contain alkaloids, phenolics, flavonoids, saponins, tannins, and terpenoids, several of which exhibit potential antioxidant activity relevant to the prevention of free radical-induced degenerative diseases. Research on the bioactive constituents of pigeon pea in Indonesia remains limited, warranting a preliminary investigation into its potential as a locally sourced functional food. This study aimed to determine the total phenolic and flavonoid content, evaluate antioxidant activity, and qualitatively identify the isoflavones genistein and daidzein in a 70% methanol extract of pigeon pea. Extraction was performed using the Ultrasound Assisted Extraction (UAE) method. Total phenolic content was determined using the Folin-Ciocalteu method, total flavonoid content using the Chang method with AlCl₃, and antioxidant activity was evaluated using the DPPH radical scavenging assay. The results showed a total phenolic content of 6.772 mg GAE/g extract and a total flavonoid content of 2.182 mg QE/g extract. The antioxidant activity assay yielded an IC₅₀ value of 593.23 ppm, which is classified as inactive compared to ascorbic acid as the positive control (IC₅₀ 6.29 ppm). This study provides preliminary scientific evidence regarding the potential of pigeon pea as a source of phenolic and flavonoid compounds that may be developed as a locally derived, health-beneficial functional food.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Gude, fenol, flavonoid, antioksidan. ------ Pigeon pea, phenolic, flavonoid, antioxidant. |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 24 Sep 2026 02:24 |
| Last Modified: | 24 Sep 2026 02:24 |
| URI: | http://repository.stfi.ac.id/id/eprint/4305 |
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