UJI AKTIVITAS ANTIOKSIDAN DAN KARAKTERISASI MINYAK ATSIRI DAUN CENGKEH MUDA DAN DAUN CENGKEH TUA (Syzygium aromaticum L.)

WINDY MEILANI, - (2026) UJI AKTIVITAS ANTIOKSIDAN DAN KARAKTERISASI MINYAK ATSIRI DAUN CENGKEH MUDA DAN DAUN CENGKEH TUA (Syzygium aromaticum L.). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Indonesia merupakan negara dengan keanekaragaman tumbuhan yang melimpah, termasuk tanaman cengkeh (Syzygium aromaticum L.) yang memiliki potensi sebagai sumber minyak atsiri. Daun cengkeh mengandung minyak atsiri sebesar 1– 4% namun perbedaan umur daun diduga memengaruhi kualitas minyak atsiri. Penelitian ini bertujuan untuk menguji karakteristik fisik dan kimia, serta aktivitas antioksidan minyak atsiri daun cengkeh muda dan daun cengkeh tua. Metode yang digunakan meliputi destilasi uap-air untuk ekstraksi minyak atsiri, karakterisasi berdasarkan parameter organoleptik, bobot jenis, indeks bias, kelarutan dalam alkohol, dan kromatografi lapis tipis serta uji aktivitas antioksidan dengan metode DPPH. Karakteristik minyak atsiri daun muda berwarna bening, bobot jenis 1,040 dan indeks bias 1,373, sedangkan minyak atsiri daun tua berwarna bening kekuningan, bobot jenis 1,036 dan indeks bias 1,424. Minyak atsiri daun tua memiliki nilai IC₅₀ 6,474 µg/mL, sedangkan daun muda memiliki IC₅₀ 12,436 µg/mL. Analisis statistik terhadap nilai IC₅₀ menunjukkan adanya perbedaan signifikan antar kelompok (p<0,05). Disimpulkan bahwa umur daun menyebabkan perbedaan karakteristik dan aktivitas antioksidan minyak atsiri daun cengkeh. Minyak atsiri daun tua lebih berpotensi sebagai antioksidan alami daripada minyak atsiri daun muda. ------- Indonesia is a country with abundant plant diversity, including clove plants (Syzygium aromaticum L.) which have the potential as a source of essential oils. Clove leaves contain 1–4% essential oil, yet leaf age is suspected to influence the oil's quality. This study aimed to examine the physical and chemical characteristics, as well as the antioxidant activity, of essential oils derived from young and mature clove leaves. The methods employed included hydro-distillation for oil extraction; characterization based on organoleptic, specific gravity, refractive index, solubility in alcohol, and thin-layer chromatography; and antioxidant activity testing with the DPPH method. The young leaf essential oil was clear, with a specific gravity of 1.040 and a refractive index of 1.373, whereas the mature leaf essential oil was yellowish-clear, with a specific gravity of 1.036 and a refractive index of 1.424. The mature leaf essential oil exhibited an IC₅₀ value of 6.474 µg/mL, while the young leaf oil showed an IC₅₀ of 12.436 µg/mL. Statistical analysis of the IC₅₀ values revealed a significant difference between the groups (p<0.05). Leaf age results in differences regarding the characteristics and antioxidant activity of clove leaf essential oil. Mature leaf essential oil holds greater potential as a natural antioxidant compared to young leaf essential oil.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	aktivitas antioksidan, DPPH, IC50, minyak atsiri, Syzygium aromaticum L. ----- antioxidant activity, DPPH, IC50, essential oil, Syzygium aromaticum L.
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	23 Sep 2026 07:28
Last Modified:	23 Sep 2026 07:28
URI:	http://repository.stfi.ac.id/id/eprint/4287

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