HANA ROHADATUL AISY, - (2026) PENETAPAN KADAR TOTAL FENOLIK, TOTAL FLAVONOID DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK KACANG KORO BENGUK (Mucuna pruriens (L.) D.C). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Kacang koro benguk (Mucuna pruriens (L.) D.C.) memiliki kandungan senyawa bioaktif seperti fenolik, flavonoid, alkaloid, tanin, saponin, triterpenoid, serta isoflavon seperti genistein dan daidzein yang berpotensi sebagai antioksidan alami. Namun, informasi mengenai kadar total fenolik, total flavonoid, serta kaitannya dengan aktivitas antioksidan ekstrak kacang koro benguk masih terbatas. Penelitian ini bertujuan menentukan kadar total fenolik, kadar total flavonoid, dan uji aktivitas antioksidan ekstrak metanol 70% kacang koro benguk. Ekstraksi dilakukan menggunakan metode ultrasound assisted extraction (UAE) dengan pelarut metanol 70%. Kadar total fenolik ditentukan dengan metode Folin–Ciocalteu menggunakan standar asam galat, dan kadar total flavonoid dengan metode AlCl₃ menggunakan standar kuersetin, sedangkan aktivitas antioksidan diuji dengan metode DPPH menggunakan asam askorbat sebagai pembanding. Hasil penelitian menunjukkan rendemen ekstraksi sebesar 8,8%, dengan kadar total fenolik sebesar 140,5686 ± 0,5883 mg GAE/g dan kadar total flavonoid sebesar 3,5310 ± 0,0506 mg QE/g. Uji aktivitas antioksidan ekstrak kacang koro benguk menunjukkan nilai IC₅₀ sebesar 47,7228 ppm, sedangkan asam askorbat memiliki IC₅₀ sebesar 6,2939 ppm. Tingginya kadar fenolik dibandingkan flavonoid diduga berkontribusi besar terhadap aktivitas antioksidan, sehingga ekstrak kacang koro benguk tergolong antioksidan yang sangat kuat. Kesimpulan pada penelitian ini bahwa ekstrak metanol 70% kacang koro benguk berpotensi sebagai sumber antioksidan alami. ------- The koro benguk bean (Mucuna pruriens (L.) D.C.) contains bioactive compounds including phenolics, flavonoids, alkaloids, tannins, saponins, triterpenoids, and isoflavones such as genistein and daidzein, which show potential as natural antioxidants. However, data on the total phenolic content, total flavonoid content, and their relationship to antioxidant activity in koro benguk bean extracts remain limited. This study aimed to determine these properties in a 70% methanol extract obtained via ultrasound-assisted extraction (UAE). Total phenolic content was assessed using the Folin–Ciocalteu method with gallic acid as standard, total flavonoid content using the AlCl₃ method with quercetin as standard, and antioxidant activity via the DPPH assay with ascorbic acid as control. Results showed an extraction yield of 8.8%, total phenolic content of 140.5686 ± 0.5883 mg GAE/g, and total flavonoid content of 3.5310 ± 0.0506 mg QE/g. The extract exhibited an IC₅₀ of 47.7228 ppm, compared to 6.2939 ppm for ascorbic acid. The higher phenolic than flavonoid content likely drives the extract's strong antioxidant activity, classifying it as a very potent antioxidant source. The conclusion of this study is that the 70% methanol extract of koro benguk beans has potential as a source of natural antioxidants.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Koro, Benguk, Fenolik, Flavonoid, Antioksidan. ----- Koro, Benguk, ,Phenolic, Flavonoid, Antioxidant |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 23 Sep 2026 07:22 |
| Last Modified: | 23 Sep 2026 07:22 |
| URI: | http://repository.stfi.ac.id/id/eprint/4286 |
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