KARAKTERISASI MOLEKULER BIOELISITOR FUNGI ENDOFIT YANG DIISOLASI DARI TANAMAN SAMBILOTO (Andrographis paniculata)

VIKA SRI RIJKI, - (2026) KARAKTERISASI MOLEKULER BIOELISITOR FUNGI ENDOFIT YANG DIISOLASI DARI TANAMAN SAMBILOTO (Andrographis paniculata). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Fungi endofit merupakan mikroorganisme yang hidup di dalam jaringan tanaman tanpa menimbulkan gejala penyakit serta berpotensi dimanfaatkan sebagai sumber bioelisitor. Pada fungi endofit yang diisolasi dari tanaman sambiloto (Andrographis paniculata), karakterisasi gen Polyketide Synthase (PKS) sebagai dasar pengembangan bioelisitor masih terbatas. Penelitian ini bertujuan untuk mengkarakterisasi secara molekuler bioelisitor fungi endofit yang diisolasi dari tanaman sambiloto berdasarkan gen PKS pada miselium aktif dan terinaktivasi. Penelitian meliputi karakterisasi morfologi, identifikasi molekuler melalui daerah Internal Transcribed Spacer (ITS), analisis BLAST dan filogenetik, desain primer gen PKS, amplifikasi gen PKS menggunakan Polymerase Chain Reaction (PCR), serta visualisasi hasil amplifikasi dengan elektroforesis gel agarosa pada sampel Larutan Miselium (LM) dan Larutan Miselium Terinaktivasi (LMT). Hasil penelitian menunjukkan bahwa isolat PD2A teridentifikasi sebagai Cladosporium sp. berdasarkan analisis BLAST (Query Coverage 100% dan E-value 0,0), serta pohon filogenetik menunjukkan kekerabatan dengan Cladosporium sp. Gen PKS terdeteksi pada sampel LM dengan pita DNA pada kisaran 500-600 bp, sedangkan pada LMT pita DNA target tampak lebih lemah. Gen PKS lebih jelas terdeteksi pada miselium aktif dibandingkan miselium terinaktivasi, sehingga isolat fungi endofit PD2A berpotensi dikembangkan sebagai sumber bioelisitor. ------- Endophytic fungi are microorganisms that inhabit plant tissues without causing disease symptoms and have potential as sources of bio-elicitors. However, molecular characterization of the Polyketide Synthase (PKS) gene in endophytic fungi isolated from Andrographis paniculata as a basis for bio-elicitor development remains limited. This study aimed to molecularly characterize bio-elicitorproducing endophytic fungi isolated from A. paniculata based on the PKS gene in active and inactivated mycelia. The study included morphological characterization, molecular identification using the Internal Transcribed Spacer (ITS) region, BLAST and phylogenetic analyses, PKS primer design, PKS gene amplification by Polymerase Chain Reaction (PCR), and visualization of PCR products by agarose gel electrophoresis using Mycelial Suspension (LM) and Inactivated Mycelial Suspension (LMT) samples. The results showed that isolate PD2A was identified as Cladosporium sp. based on BLAST analysis (100% query coverage and E-value of 0.0), and phylogenetic analysis confirmed its close relationship with Cladosporium sp. The PKS gene was detected in the LM sample, showing a DNA band at approximately 500–600 bp, whereas the target band in the LMT sample was weaker. The PKS gene was more clearly detected in active mycelium than in inactivated mycelium, indicating that the PD2A endophytic fungal isolate has potential for development as a bio-elicitor source.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Cladosporium sp., fungi endofit, gen polyketide synthase, sambiloto, sekuensing ITS. ---- Cladosporium sp., endophytic fungi, ITS sequencing, Polyketide Synthase gene, sambiloto
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	23 Sep 2026 07:19
Last Modified:	23 Sep 2026 07:19
URI:	http://repository.stfi.ac.id/id/eprint/4285

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