MISLAMAH, - (2026) OPTIMASI METODE EKSTRAKSI DARI AKAR PASAK BUMI (Eurycoma longifolia jack.) MENGGUNAKAN RESPONSE SURFACE METHODOLOGY (RSM). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Akar pasak bumi (Eurycoma longifolia Jack.) merupakan tanaman obat yang mengandung senyawa quassinoid, terutama eurycomanone, yang berperan dalam berbagai aktivitas farmakologis. Keberhasilan proses ekstraksi dipengaruhi oleh kondisi ekstraksi, terutama konsentrasi pelarut dan waktu ekstraksi. Penelitian ini bertujuan mengetahui pengaruh kedua faktor tersebut terhadap rendemen ekstrak dan kadar eurycomanone, menentukan faktor yang paling berpengaruh, menyusun model matematis menggunakan Response Surface Methodology (RSM), serta memperoleh kondisi ekstraksi optimum. Ekstraksi dilakukan dengan metode maserasi menggunakan rasio pelarut terhadap simplisia 1:10 (b/v). Optimasi menggunakan RSM dengan rancangan Central Composite Design (CCD) yang melibatkan konsentrasi etanol 0%, 48%, dan 96% serta waktu ekstraksi 1, 13, dan 24 jam. Respon yang diamati meliputi rendemen ekstrak dan kadar eurycomanone yang ditetapkan menggunakan Kromatografi Lapis Tipis (KLT)-densitometri. Analisis data dilakukan menggunakan ANOVA, General Linear Model, regresi, dan optimasi RSM. Hasil penelitian menunjukkan bahwa konsentrasi pelarut dan waktu ekstraksi berpengaruh signifikan terhadap rendemen ekstrak dan kadar eurycomanone (p<0,05). Model RSM menghasilkan nilai R² sebesar 97,37% untuk rendemen dan 59,29% untuk kadar eurycomanone. Kondisi optimum diperoleh pada etanol 96% selama 24 jam dengan nilai desirability 0,969247, menghasilkan prediksi rendemen 6,8672% dan kadar eurycomanone 1,5671%. --------- Akar Pasak Bumi (Eurycoma longifolia Jack.) is a medicinal plant containing quassinoid compounds, particularly eurycomanone, which plays a role in various pharmacological activities. The success of the extraction process is influenced by extraction conditions, particularly solvent concentration and extraction time. This study aims to determine the effect of these two factors on extract yield and eurycomanone content, identify the most influential factor, develop a mathematical model using Response Surface Methodology (RSM), and determine the optimal extraction conditions. Extraction was performed using the maceration method with a solvent-to-herb ratio of 1:10 (w/v). Optimization was performed using RSM with a Central Composite Design (CCD) involving ethanol concentrations of 0%, 48%, and 96% and extraction times of 1, 13, and 24 hours. The observed responses included extract yield and eurycomanone content, which were determined using Thin-Layer Chromatography (TLC)-densitometry. Data analysis was performed using ANOVA, the General Linear Model, regression, and RSM optimization. The results showed that solvent concentration and extraction time had a significant effect on extract yield and eurycomanone content (p < 0.05). The RSM model yielded an R² value of 97.37% for yield and 59.29% for eurycomanone content. Optimal conditions were obtained using 96% ethanol for 24 hours with a desirability value of 0.969247, resulting in a predicted yield of 6.8672% and a predicted eurycomanone content of 1.5671%.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Eurycoma longifolia Jack., akar pasak bumi, eurycomanone, ekstraksi, Response Surface Methodology (RSM), KLT-densitometri. ------- Eurycoma longifolia Jack., akar pasak bumi, eurycomanone, Extraction, Response Surface Methodology (RSM), KLT-densitometri |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 23 Sep 2026 03:12 |
| Last Modified: | 23 Sep 2026 03:12 |
| URI: | http://repository.stfi.ac.id/id/eprint/4272 |
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