OPTIMASI METODE MASERASI KAYU SECANG (CAESALPINIA SAPPAN L.) MENGGUNAKAN METODE RESPONSE SURFACE METHODOLOGY (RSM)

ADINDA PUTRI, - (2026) OPTIMASI METODE MASERASI KAYU SECANG (CAESALPINIA SAPPAN L.) MENGGUNAKAN METODE RESPONSE SURFACE METHODOLOGY (RSM). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Kayu secang (Caesalpinia sappan L.) merupakan tanaman obat yang mengandung brazilin sebagai senyawa utama dengan berbagai aktivitas farmakologi. Keberhasilan proses ekstraksi dipengaruhi oleh waktu ekstraksi dan konsentrasi pelarut sehingga diperlukan optimasi untuk memperoleh rendemen dan kadar brazilin yang optimum. Penelitian ini bertujuan mengoptimasi metode maserasi kayu secang menggunakan Response Surface Methodology (RSM) dengan desain Face Centered Central Composite Design (FCCD). Faktor yang dioptimasi meliputi waktu maserasi (1, 13, dan 24 jam) dan konsentrasi etanol (0%, 48%, dan 96%), sedangkan respon yang diamati adalah rendemen dan kadar brazilin. Kadar brazilin ditentukan menggunakan metode Kromatografi Lapis Tipis (KLT)- densitometri. Hasil analisis menunjukkan bahwa model kuadratik memberikan kecocokan yang baik terhadap data dengan nilai koefisien determinasi (R²) sebesar 95,67% untuk rendemen dan 83,38% untuk kadar brazilin. Kondisi optimum diperoleh pada waktu maserasi 13 jam dan konsentrasi etanol 48%, dengan prediksi rendemen sebesar 10,31% dan kadar brazilin 9,6%, serta nilai composite desirability sebesar 0,921. Hasil penelitian menunjukkan bahwa pendekatan RSM mampu mengoptimalkan proses ekstraksi kayu secang secara efisien sehingga menghasilkan ekstrak dengan rendemen dan kadar brazilin yang optimal. -------- Caesalpinia sappan L. is a medicinal plant containing brazilin as its major bioactive compound with various pharmacological activities. The extraction process is influenced by extraction time and solvent concentration; therefore, optimization is required to obtain the highest extraction yield and brazilin content. This study aimed to optimize the maceration method of Caesalpinia sappan L. using Response Surface Methodology (RSM) with a Face Centered Central Composite Design (FCCD). The investigated factors were extraction time (1, 13, and 24 h) and ethanol concentration (0%, 48%, and 96%), while the responses were extraction yield and brazilin content. Brazilin content was determined using Thin Layer Chromatography (TLC)-densitometry. The quadratic model showed good agreement with the experimental data, with coefficients of determination (R²) of 95,67% for extraction yield and 83,38% for brazilin content. The optimum extraction condition was achieved at 13 h of maceration using 48% ethanol, producing a predicted extraction yield of 10,31% and brazilin content of 9,6%, with a composite desirability value of 0,921. These findings demonstrate that RSM is an effective approach for optimizing the extraction process of Caesalpinia sappan L. to obtain extracts with optimal yield and brazilin content.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Caesalpinia sappan L., brazilin, KLT-densitometri, maserasi, Response Surface Methodology. ------- Caesalpinia sappan L., brazilin, maceration, Response Surface Methodology, TLC-densitometry.
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	23 Sep 2026 02:23
Last Modified:	23 Sep 2026 02:23
URI:	http://repository.stfi.ac.id/id/eprint/4269

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