KURNIA PUTERI AGUSTINA, - (2026) UJI SITOTOKSISITAS EKSTRAK ETANOL DAUN SINGAWALANG (Petiveria alliacea L.) TERHADAP LINI SEL KANKER PARU-PARU A549 DENGAN METODE RESAZURIN. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Kanker paru-paru merupakan salah satu penyebab utama kematian akibat kanker di dunia, sehingga diperlukan pengembangan kandidat antikanker baru yang berasal dari bahan alam. Daun singawalang (Petiveria alliacea L.) diketahui mengandung berbagai metabolit sekunder yang berpotensi memiliki aktivitas sitotoksik seperti dibenzil disulfida, dibenzil trisulfida, serta flavonoid, namun pengujiannya terhadap sel kanker paru-paru A549 masih terbatas. Penelitian ini bertujuan untuk mengetahui aktivitas sitotoksik ekstrak etanol daun singawalang terhadap lini sel kanker paru-paru A549 serta menentukan nilai IC₅₀. Penelitian dilakukan secara eksperimental menggunakan ekstrak etanol yang diperoleh melalui metode maserasi dengan etanol 70%, kemudian dilakukan karakterisasi simplisia, skrining fitokimia, dan pengujian sitotoksisitas terhadap sel A549 dengan variasi konsentrasi 7,81–1000 µg/mL. Viabilitas sel diukur menggunakan reagen PrestoBlue™ dan nilai IC50 dihitung berdasarkan kurva dose-response menggunakan GraphPad Prism. Hasil penelitian menunjukkan rendemen ekstrak sebesar 10,92% dan ekstrak mengandung alkaloid, fenolik, flavonoid, tanin, saponin, serta steroid. Hasil uji sitotoksisitas menunjukkan bahwa ekstrak etanol daun singawalang menurunkan viabilitas sel A549 secara dose-dependent. Nilai IC50 yang diperoleh sebesar 617,20 µg/mL, sehingga berdasarkan klasifikasi aktivitas sitotoksik termasuk dalam kategori tidak sitotoksik terhadap sel kanker paru A549. Kesimpulan dari penelitian ini, ekstrak daun singawalang tidak memiliki aktivitas sitotoksik terhadap kanker paru-paru A549. ----- Lung cancer is one of the leading causes of cancer-related mortality worldwide; therefore, the development of new anticancer candidates derived from natural products is needed. Singawalang leaves (Petiveria alliacea L.) are known to contain various secondary metabolites with potential cytotoxic activity, such as dibenzyl disulfide, dibenzyl trisulfide, and flavonoids; however, studies evaluating their activity against A549 lung cancer cells are still limited. This study aimed to determine the cytotoxic activity of the ethanolic extract of singawalang leaves against the A549 lung cancer cell line and to determine its IC₅₀ value. The study was conducted experimentally using an ethanolic extract obtained by the maceration method with 70% ethanol, followed by crude drug characterization, phytochemical screening, and cytotoxicity testing against A549 cells at concentrations ranging from 7.81–1000 µg/mL. Cell viability was measured using the PrestoBlue™ reagent, and the IC₅₀ value was calculated based on the dose– response curve using GraphPad Prism. The results showed that the extraction yield was 10.92%, and the extract contained alkaloids, phenolics, flavonoids, tannins, saponins, and steroids. The cytotoxicity test results showed that the ethanolic extract of singawalang leaves reduced the viability of A549 cells in a dose-dependent manner. The obtained IC₅₀ value was 617.20 µg/mL; therefore, based on the cytotoxic activity classification, it was categorized as non-cytotoxic against A549 lung cancer cells. In conclusion, the ethanolic extract of singawalang leaves did not exhibit cytotoxic activity against A549 lung cancer cells.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Sitotoksisitas, Singawalang, Kanker, Paru-Paru. ------ Cytotoxicity, Singawalang Leaves, Lung, Cancer |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 22 Sep 2026 06:12 |
| Last Modified: | 22 Sep 2026 06:12 |
| URI: | http://repository.stfi.ac.id/id/eprint/4265 |
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