SALSA SEVIRA, - (2026) UJI SITOTOKSISITAS EKSTRAK ETANOL HERBA RUMPUT MUTIARA (Hedyotis corymbosa (L.) Lam.) TERHADAP LINI SEL KANKER PARU-PARU A549 DENGAN METODE RESAZURIN. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Kanker paru-paru merupakan salah satu jenis kanker dengan tingkat kematian tertinggi sehingga diperlukan pengembangan alternatif terapi berbasis bahan alam dengan efek samping yang relatif rendah. Herba rumput mutiara (Hedyotis corymbosa (L.) Lam.) mengandung berbagai senyawa bioaktif seperti asam ursolat dan fenolik yang berpotensisebagai antikanker. Penelitian ini bertujuan mengetahui aktivitas sitotoksik dan menentukan nilai IC₅₀ ekstrak etanol herba rumput mutiara terhadap lini sel kanker paruparu A549 menggunakan metode Resazurin. Ekstrak diperoleh dari ekstraksi secara maserasi menggunakan pelarut etanol 70%, kemudian dilakukan karakterisasi simplisia, skrining fitokimia dan uji sitotoksik dengan variasi konsentrasi ekstrak yang digunakan sebanyak 8 konsentrasi yakni 1000 hingga 7,81 µg/mL dengan nilai IC₅₀ sebagai parameter yang ditentukan menggunakan software GraphPad Prism. Hasil skrining fitokimia menunjukan bahwa ekstrak mengandung alkaloid, fenolik, flavonoid, tanin, saponin, steroid dan triterpenoid. Nilai IC₅₀ ekstrak etanol herba rumput mutiara yang diperoleh sebesar 614,30 µg/mL dengan kategori tidak sitotoksik. Kesimpulan dari penelitian ini adalah ekstrak herba rumput mutiara bersifat tidak toksik terhadap sel kanker paru-paru A549. ------ Lung cancer is one of the types of cancer with the highest mortality rates; therefore, the development of alternative therapies derived from natural products with relatively low side effects is needed. Pearl grass (Hedyotis corymbosa (L.) Lam.) contains various bioactive compounds, such as ursolic acid and phenolic compounds, which have potential anticancer activity. This study aimed to evaluate the cytotoxic activity and determine the IC₅₀ value of the ethanolic extract of pearl grass against the A549 lung cancer cell line using the resazurin assay. The extract was obtained through maceration using 70% ethanol, followed by crude drug characterization, phytochemical screening, and cytotoxicity testing. The cytotoxicity assay was performed using eight extract concentrations ranging from 1,000 to 7,81 µg/mL, and the IC₅₀ value was determined using GraphPad Prism software. The phytochemical screening results showed that the extract contained alkaloids, phenolic compounds, flavonoids, tannins, saponins, steroids, and triterpenoids. The IC₅₀ value of the ethanolic extract of pearl grass against A549 cells was 614,30 µg/mL, indicating that the extract was categorized as non-cytotoxic against A549 cells. This study concluded that the ethanolic extract of pearl grass was non-cytotoxic against A549 lung cancer cells.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | sitotoksisitas, kanker, paru-paru, rumput mutiara. ------ cytotoxicity, cancer, lung, pearl grass |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 22 Sep 2026 03:18 |
| Last Modified: | 22 Sep 2026 03:18 |
| URI: | http://repository.stfi.ac.id/id/eprint/4262 |
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