RAHEL MARGARENTA SINABUTAR, - (2026) OPTIMASI IMUNOSENSOR ELEKTROKIMIA BERBASIS NANOBODI PADA Screen-Printed Gold Electrode (SPGE) SEBAGAI DETEKTOR KORTISOL. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
![[img]](http://repository.stfi.ac.id/style/images/fileicons/text.png) |
Text
S_PSSF_A221072_Title.pdf Download (3MB) |
|
Text
S_PSSF_A221072_Chapter1.pdf Restricted to Repository staff only Download (178kB) |
|
Text
S_PSSF_A221072_Chapter2.pdf Restricted to Repository staff only Download (224kB) |
|
Text
S_PSSF_A221072_Chapter3.pdf Restricted to Repository staff only Download (219kB) |
|
Text
S_PSSF_A221072_Chapter4.pdf Restricted to Repository staff only Download (715kB) |
|
Text
S_PSSF_A221072_Chapter5.pdf Restricted to Repository staff only Download (122kB) |
|
Text
S_PSSF_A221072_Appendix.pdf Restricted to Repository staff only Download (609kB) |
Abstract
Stres merupakan masalah kesehatan global yang berkaitan dengan peningkatan hormon kortisol sebagai biomarker utama respons stres. Penelitian ini bertujuan mengembangkan imunosensor elektrokimia berbasis nanobodi kortisol (NbCor) pada platform Screen-Printed Gold Electrode (SPGE) untuk deteksi kortisol secara sensitif dan selektif. Nanobodi diimobilisasi pada permukaan SPGE, kemudian dikarakterisasi menggunakan Cyclic Voltammetry (CV), sedangkan respons sensor dievaluasi menggunakan Differential Pulse Voltammetry (DPV). Optimasi dilakukan pada konsentrasi NbCor 1; 5; dan 10 mg/mL untuk memperoleh kondisi imobilisasi optimum. Hasil menunjukkan bahwa konsentrasi NbCor 10 mg/mL menghasilkan nilai inhibisi tertinggi sebesar 70,16%. Sensor memberikan respons linear terhadap konsentrasi kortisol dengan persamaan regresi y = −36,667x − 44,828 dan nilai R² sebesar 0,9445. Nilai Limit of Detection (LoD) dan Limit of Quantification (LoQ) masing-masing sebesar 3,58 ng/mL dan 10,85 ng/mL. Berdasarkan hasil tersebut, dapat disimpulkan bahwa imunosensor elektrokimia berbasis SPGE–NbCor berhasil dikembangkan dan dioptimasi untuk deteksi kortisol dengan kinerja analitik yang baik, ditunjukkan oleh keberhasilan imobilisasi nanobodi, respons yang linear terhadap peningkatan konsentrasi kortisol serta memiliki sensitivitas yang memadai, sehingga berpotensi untuk pengembangan sistem deteksi kortisol berbasis elektrokimia. ------- Stress is a global health concern associated with elevated cortisol levels, the primary biomarker of the physiological stress response. This study aimed to develop an electrochemical immunosensor based on cortisol nanobodies (NbCor) using a Screen-Printed Gold Electrode (SPGE) platform for sensitive and selective cortisol detection. The nanobodies were immobilized on the SPGE surface and characterized using Cyclic Voltammetry (CV), while the sensor response was evaluated by Differential Pulse Voltammetry (DPV). The NbCor concentration was optimized at 1; 5; and 10 mg/mL to determine the optimal immobilization condition. The results showed that the optimum NbCor concentration was 10 mg/mL, yielding the highest inhibition value of 70.16%. The sensor exhibited a linear response to increasing cortisol concentrations with the regression equation y = −36.667x − 44.828 and a coefficient of determination (R²) of 0.9445. The Limit of Detection (LoD) and Limit of Quantification (LoQ) were 3.58 ng/mL and 10.85 ng/mL, respectively. Based on these findings, the SPGE–NbCor electrochemical immunosensor was successfully developed and optimized for cortisol detection, demonstrating good analytical performance through successful nanobody immobilization, a linear response to increasing cortisol concentrations, and adequate sensitivity. Therefore, the developed sensor shows potential for further development as an electrochemical cortisol detection system.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | kortisol, nanobodi, SPGE, biosensor, elektrokimia. ---- cortisol, nanobody, SPGE, electrochemical, biosensor. |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH426 Genetics |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 19 Sep 2026 02:51 |
| Last Modified: | 19 Sep 2026 02:51 |
| URI: | http://repository.stfi.ac.id/id/eprint/4251 |
Actions (login required)
 |
View Item |
