APLIKASI SOLID PHASE EXTRACTION (SPE) UNTUK PEMURNIAN SENYAWA PIPERIN MENGGUNAKAN METODE SPEKTROFOTOMETER UV-VISIBEL DAN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

MUHAMAD AZRUL, - (2025) APLIKASI SOLID PHASE EXTRACTION (SPE) UNTUK PEMURNIAN SENYAWA PIPERIN MENGGUNAKAN METODE SPEKTROFOTOMETER UV-VISIBEL DAN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Piperin merupakan senyawa utama dalam buah lada (Piper nigrum L.) yang memiliki berbagai aktivitas farmakologis seperti antiinflamasi, analgesik, dan hepatoprotektan. Proses isolasi piperin memiliki keterbatasan yang disebabkan oleh adanya senyawa pengotor serta waktu ekstraksi yang relatif panjang, sehingga efisiensi pemurnian menjadi rendah. Penelitian ini bertujuan untuk mengetahui efektivitas metode Solid Phase Extraction (SPE) dalam meningkatkan kemurnian isolat piperin yang dianalisis menggunakan Spektrofotometer UV-Visibel dan High Performance Liquid Chromatography (HPLC). Tahapan penelitian meliputi preparasi larutan isolat piperin 100 ppm, optimasi sistem SPE dengan kartrid C-18, serta analisis kadar dan kemurnian sebelum dan sesudah pemurnian menggunakan HPLC dengan sistem fase gerak Asetonitril : Buffer asetat 2% (60 : 40) dengan laju alir 10 µL/menit. Berdasarkan penelitian, persen area yang didapatkan meningkat dari 96,32% sebelum SPE menjadi 97,97% setelah tahap pemisahan. Dengan demikian, metode SPE terbukti efektif sebagai teknik pemurnian sederhana, cepat, dan efisien untuk meningkatkan kemurnian piperin dalam isolat piperin. ------- Piperine is the main compound in pepper fruit (Piper nigrum L.) which has various pharmacological activities such as anti-inflammatory, analgesic and hepatoprotectant. The piperine isolation process has limitations caused by the presence of impurities and relatively long extraction times, resulting in low purification efficiency. This research aims to determine the effectiveness of the Solid Phase Extraction (SPE) method in increasing the purity of piperine isolates which were analyzed using a UV-Visible Spectrophotometer and High Performance Liquid Chromatography (HPLC). The research stages included preparation of a 100 ppm piperine isolate solution, optimization of the SPE system with a C-18 cartridge, as well as content and purity analysis before and after purification using HPLC with the mobile phase system Acetonitrile: 2% acetate buffer (60 : 40) with a flow rate of 10 µL/minute. Based on research, the percent area obtained increased from 96.32% before SPE to 97.97% after the separation stage. Thus, the SPE method was proven to be effective as a simple, fast and efficient purification technique to increase the purity of piperine in piperine isolates.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Piperin, Solid Phase Extraction (SPE), High Performance Liquid Chromatography (HPLC), Kemurnian. ------- Piperine, Solid Phase Extraction (SPE), High Performance Liquid Chromatography (HPLC), Purity.
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	20 Nov 2025 04:51
Last Modified:	20 Nov 2025 04:51
URI:	http://repository.stfi.ac.id/id/eprint/3534

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