FARHAN SHIDIK, - (2025) PENGARUH ZAT PENGATUR TUMBUH NAA DAN BAP TERHADAP KADAR ANDROGRAFOLID BERDASARKAN WAKTU PANEN PADA KULTUR TUNAS SAMBILOTO (Andrographis paniculate Ness). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Andrografolid merupakan senyawa bioaktif utama dalam tanaman sambiloto (Andrographis paniculata Ness.). Kandungan andrografolid dalam tanaman dapat dipengaruhi oleh faktor fisiologis seperti umur tanaman, bagian organ yang digunakan, dan perlakuan zat pengatur tumbuh (ZPT). Teknik kultur jaringan dan manipulasi hormon diperlukan dalam upaya optimalisasi kadar andrografolid. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi zat pengatur tumbuh NAA (Naphthalene Acetic Acid) dan BAP (6-Benzylaminopurine) terhadap pertumbuhan serta kadar Andrografolid berdasarkan waktu panen pada kultur tunas sambiloto (Andrographis paniculata Ness). Metode yang digunakan meliputi teknik kultur jaringan pada media Murashige dan Skoog (MS) dengan penambahan kombinasi NAA dan BAP dengan variasi konsentrasi berbeda yaitu 1:1,1:2, 2:1 serta MS sebagai kontrol. Analisis kadar andrografolid dilakukan menggunakan instrumen kromatografi cair kinerja tinggi (KCKT) pada bagian daun, batang, dan akar. Hasil penelitian menunjukkan bahwa variasi konsentrasi NAA dan BAP berpengaruh negatif terhadap pertumbuhan tunas. Hasil analisis KCKT menunjukan kadar tertinggi Andrografolid pada kontrol secara konsisten ditemukan pada bagian daun di minggu pertama sebesar 62,1%. Daun merupakan lokasi utama biosintesis dan akumulasi awal metabolit Andrografolid, bertambahnya waktu pada minggu kedua hingga minggu ketiga menunjukkan penurunan kadar. Uji statistik ANOVA menunjukkan bahwa waktu panen memberikan pengaruh yang signifikan terhadap kadar Andrografolid pada tiga bagian tanaman (daun 0,049, batang 0,040, dan akar 0,002). ------- Andrographolide is the main bioactive compound found in Andrographis paniculata (Nees). The andrographolide content in the plant can be influenced by physiological factors such as plant age, plant organ used, and the application of plant growth regulators (PGRs). Tissue culture techniques and hormonal manipulation are required to optimize andrographolide levels. This study aimed to determine the effect of the combination of plant growth regulators NAA (Naphthalene Acetic Acid) and BAP (6-Benzylaminopurine) on the growth and andrographolide content based on harvest time in Andrographis paniculata shoot cultures. The method used involved tissue culture on Murashige and Skoog (MS) medium supplemented with combinations of NAA and BAP at different concentration ratios, namely 1:1, 1:2, and 2:1, with MS medium serving as the control. The andrographolide content was analyzed using High-Performance Liquid Chromatography (HPLC) on leaves, stems, and roots. The results showed that variations in NAA and BAP concentrations had a negative effect on shoot growth. HPLC analysis revealed that the highest andrographolide content in the control treatment was consistently found in the leaves during the first week, reaching 62.1%. The leaves served as the primary site for andrographolide biosynthesis and early metabolite accumulation. However, an extended culture period from the second to the third week showed a decline in andrographolide levels. Statistical analysis using ANOVA indicated that harvest time had a significant effect on andrographolide content in all three plant parts (leaves p = 0.049, stems p = 0.040, and roots p = 0.002).
| Item Type: | Thesis (Skripsi) |
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| Uncontrolled Keywords: | Kultur jaringan, Andrographis paniculata, ZPT, Andrografolid, KCKT. ------ Tissue culture, Andrographis paniculata, plant growth regulators, andrographolide, HPLC |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 19 Nov 2025 07:58 |
| Last Modified: | 19 Nov 2025 07:58 |
| URI: | http://repository.stfi.ac.id/id/eprint/3506 |
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