AKTIVITAS ANTIOKSIDAN DAN NILAI SUN PROTECTION FACTOR (SPF) EKSTRAK DAN FRAKSI DAUN JERUK GERGA (Citrus nobilis L.)

CLARA ULINTA SILABAN, - (2025) AKTIVITAS ANTIOKSIDAN DAN NILAI SUN PROTECTION FACTOR (SPF) EKSTRAK DAN FRAKSI DAUN JERUK GERGA (Citrus nobilis L.). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Radikal bebas berperan dalam memicu penyakit degeneratif dan kerusakan kulit akibat paparan sinar ultraviolet (UV). Antioksidan berfungsi menetralkan radikal bebas, sedangkan tabir surya melindungi kulit dengan menghambat penetrasi radiasi UV. Daun jeruk gerga (Citrus nobilis L.) diketahui mengandung flavonoid, fenol, dan vitamin C yang berpotensi sebagai sumber antioksidan alami sekaligus agen pelindung UV. Penelitian ini bertujuan mengetahui aktivitas antioksidan dan nilai Sun Protection Factor (SPF) dari ekstrak etanol serta fraksi daun jeruk gerga. Ekstraksi dilakukan dengan metode maserasi menggunakan etanol 96%, kemudian difraksinasi dengan n-heksan, etil asetat, dan air. Aktivitas antioksidan ditentukan menggunakan metode DPPH, sedangkan nilai SPF diukur secara in vitro dengan spektrofotometer UV-Vis. Hasil penelitian menunjukkan ekstrak etanol memiliki aktivitas antioksidan sangat kuat (IC₅₀ 16,04 ppm). Fraksi etil asetat dan air juga tergolong sangat kuat (IC₅₀ 40,58 dan 48,18 ppm), sedangkan fraksi n-heksan termasuk kuat (IC₅₀ 52,68 ppm). Uji SPF menunjukkan seluruh sampel berada pada kategori proteksi ultra, dengan nilai tertinggi pada fraksi air 500 ppm (45,71), diikuti ekstrak etanol (41,12) dan fraksi etil asetat 100 ppm (34,59). Kesimpulannya, daun jeruk gerga berpotensi dikembangkan sebagai sumber antioksidan alami sekaligus tabir surya efektif. ----- Free radicals play a role in triggering degenerative diseases and skin damage due to exposure to ultraviolet (UV) rays. Antioxidants neutralize free radicals, while sunscreen protects the skin by inhibiting the penetration of UV radiation. Gerga orange leaves (Citrus nobilis L.) are known to contain flavonoids, phenols, and vitamin C, which have the potential to be a source of natural antioxidants as well as UV protection agents. This study aims to determine the antioxidant activity and Sun Protection Factor (SPF) value of ethanol extracts and fractions of bitter orange leaves. Extraction was performed using the maceration method with 96% ethanol, followed by fractionation with n-hexane, ethyl acetate, and water. Antioxidant activity was determined using the DPPH method, while the SPF value was measured in vitro using UV-Vis spectrophotometer. The results showed that the ethanol extract had very strong antioxidant activity (IC₅₀ 16.04 ppm). The ethyl acetate and water fractions were also very strong (IC₅₀ 40.58 and 48.18 ppm), while the n-hexane fraction was strong (IC₅₀ 52.68 ppm). The SPF test showed that all samples were in the ultra-protection category, with the highest value in the 500 ppm water fraction (45.71), followed by the ethanol extract (41.12) and the 100 ppm ethyl acetate fraction (34.59). In conclusion, gerga orange leaves have the potential to be developed as a source of natural antioxidants and effective sunscreen.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Jeruk gerga, antioksidan, IC₅₀, Sun Protection Factor (SPF). ----- Gerga orange, antioxidants, IC₅₀, Sun Protection Factor (SPF)
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	19 Nov 2025 01:52
Last Modified:	19 Nov 2025 01:52
URI:	http://repository.stfi.ac.id/id/eprint/3477

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