PENENTUAN KADAR FENOLIK, FLAVONOID TOTAL DAN AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN JERUK GERGA (Citrus nobilis L.)

USWATUN RIZKI NUR AULIA, - (2025) PENENTUAN KADAR FENOLIK, FLAVONOID TOTAL DAN AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN JERUK GERGA (Citrus nobilis L.). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Infeksi bakteri non patogen seperti Staphylococcus epidermidis dan Escherichia coli masih menjadi masalah kesehatan yang sering ditemui. Penggunaan antibiotik sintetis secara berlebihan dapat menyebabkan resistensi, sehingga diperlukan alternatif antibakteri dari bahan alam. Salah satu tanaman yang berpotensi adalah daun jeruk gerga (Citrus nobilis L.) yang diketahui mengandung senyawa bioaktif seperti fenolik dan flavonoid. Penelitian ini bertujuan untuk mengetahui kadar fenolik total dan flavonoid total ekstrak etanol daun jeruk gerga serta menguji aktivitas antibakterinya terhadap S. epidermidis dan E. coli. Ekstraksi dilakukan dengan metode maserasi menggunakan etanol 70%. Penetapan kadar fenolik total menggunakan metode Folin-Ciocalteu dengan standar asam galat, sedangkan kadar flavonoid total ditentukan menggunakan metode AlCl₃ dengan standar kuersetin. Aktivitas antibakteri diuji menggunakan metode difusi sumuran dengan kontrol negatif DMSO 10% dan kontrol positif amoksisilin. Hasil penelitian menunjukkan bahwa ekstrak etanol daun jeruk gerga mengandung fenolik total sebesar 57,198 ± 0,027 mg GAE/g ekstrak dan flavonoid total sebesar 32,398 ± 0,015 mg QE/g ekstrak. Pada konsentrasi 100% ekstrak mampu menghambat pertumbuhan S. epidermidis dengan zona hambat 13,933 ± 0,715 mm, sedangkan E. coli sebesar 9,417 ± 0,202 mm. Dengan demikian daun jeruk gerga berpotensi dikembangkan sebagai antibakteri alami. ----- Non pathogenic bacterial infections such as Staphylococcus epidermidis and Escherichia coli are still a frequently encountered health problem. Excessive use of synthetic antibiotics can cause resistance, so alternative antibacterials from natural ingredients are needed. One plant that has potential is gerga orange leaves (Citrus nobilis L.) which is known to contain bioactive compounds such as phenolics and flavonoids. This study aims to determine the total phenolic and total flavonoid levels of ethanol extract of Gerga orange leaves and to test its antibacterial activity against S. epidermidis and E. coli. Extraction was carried out using the maceration method using ethanol 70%. Total phenolic content was determined using the Folin-Ciocalteu method with a gallic acid standard, while total flavonoid content was determined using the AlCl₃ method with a quercetin standard. Antibacterial activity was tested using the well diffusion method with a negative control of 10% DMSO and a positive control of amoxicillin. The results showed that the ethanol extract of Gerga orange leaves contained total phenolics of 57.198 ± 0.027 mg GAE/g extract and total flavonoids of 32.398 ± 0.015 mg QE/g extract. At a concentration of 100% the extract was able to inhibit the growth of S. epidermidis with an inhibition zone of 13.933 ± 0.715 mm, while E. coli was 9.417 ± 0.202 mm. Thus, Gerga lime leaves have the potential to be developed as a natural antibacterial.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Jeruk gerga, fenolik, flavonoid, antibakteri. ------ Gerga orange, phenolics, flavonoids, antibacterial.
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	18 Nov 2025 02:48
Last Modified:	18 Nov 2025 02:48
URI:	http://repository.stfi.ac.id/id/eprint/3468

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