PATRICK TANUWIJAYA, - (2025) ANALISIS KADAR MALONDIALDEHYDE (MDA) DARAH PENYANDANG TALASEMIA MAYOR DAN MINOR MENGGUNAKAN METODE THIOBARBITURIC ACID REACTIVE SUBSTANCES (TBARS). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
![[img]](http://repository.stfi.ac.id/style/images/fileicons/text.png) |
Text
S_PSSF_A212011_Title.pdf Download (628kB) |
|
Text
S_PSSF_A212011_Chapter1.pdf Restricted to Repository staff only Download (201kB) |
|
Text
S_PSSF_A212011_Chapter2.pdf Restricted to Repository staff only Download (282kB) |
|
Text
S_PSSF_A212011_Chapter3.pdf Restricted to Repository staff only Download (201kB) |
|
Text
S_PSSF_A212011_Chapter4.pdf Restricted to Repository staff only Download (256kB) |
|
Text
S_PSSF_A212011_Chapter5.pdf Restricted to Repository staff only Download (221kB) |
|
Text
S_PSSF_A212011_Appendix.pdf Restricted to Repository staff only Download (390kB) |
Abstract
Talasemia merupakan kelainan genetik yang diturunkan antargenerasi melalui garis keturunan, sehingga prevalensinya terus meningkat di populasi. Kondisi ini menyebabkan gangguan produksi hemoglobin yang menimbulkan anemia kronis, hemolisis, dan penumpukan zat besi. Akumulasi zat besi bebas memicu pembentukan radikal bebas yang menyebabkan stres oksidatif dan peroksidasi lipid, menghasilkan malondialdehyde (MDA) sebagai produk akhir. Penelitian ini bertujuan menganalisis kadar MDA plasma pada tiga kelompok: talasemia mayor, talasemia minor, dan individu normal menggunakan metode Thiobarbituric Acid Reactive Substances (TBARS). Plasma dipreparasi dengan trichoroacetic acid – Thiobarbituric Acid, dipanaskan 100 °C selama 20 menit, disentrifugasi, lalu diukur pada 532 nm. Kuantisasi dilakukan menggunakan kurva baku Tetraetoksipropan (y = 0.1849x – 0.0019; R² = 0.9921). Hasil menunjukkan rerata MDA meningkat berturut-turut pada kelompok normal (≈ 1,56 ppm), minor (≈ 2,95 ppm), dan mayor (≈ 6,47 ppm) dengan perbedaan bermakna (p < 0,05). Temuan ini menegaskan peran MDA sebagai penanda kerusakan oksidatif pada talasemia dan menunjukkan bahwa metode TBARS merupakan teknik sederhana, terjangkau, dan sensitif yang berpotensi digunakan dalam deteksi dini carrier guna mencegah pewarisan penyakit ke generasi berikutnya. ------- Thalassemia is an genetic disorder that can be transmitted across generations through hereditary lines, leading to an increasing prevalence within populations. This condition causes impaired hemoglobin synthesis, resulting in chronic anemia, hemolysis, and iron overload. Excess free iron promotes the generation of reactive oxygen species, inducing oxidative stress and lipid peroxidation that produce malondialdehyde (MDA) as a final product. This study aimed to analyze plasma MDA levels among three groups: Thalassemia major, minor, and healthy individuals using the Thiobarbituric Acid Reactive Substances (TBARS) method. Plasma samples were treated with trichoroacetic acid – Thiobarbituric Acid, heated at 100 °C for 20 minutes, centrifuged, and measured at 532 nm. Quantification was performed using a Tetraetoksipropan calibration curve (y = 0.1849x – 0.0019; R² = 0.9921). The results showed a consistent gradient in mean MDA levels: lowest in the normal group (≈1.56 ppm), followed by thalassemia minor (≈2.95 ppm), and highest in Thalassemia major (≈6.47 ppm), with significant differences among groups (p < 0.05). These findings reinforce MDA’s role as a biomarker of oxidative damage in thalassemia and demonstrate that the TBARS method is simple, affordable, and sensitive—holding potential as early screening tool for carriers to prevent transmission of the disease to future generations.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | talasemia, MDA, TBARS, stres oksidatif, TEP. ----- thalassemia, malondialdehyde, TBARS, oxidative stress, TEP calibration. |
| Subjects: | Q Science > QD Chemistry R Medicine > R Medicine (General) |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 17 Nov 2025 07:13 |
| Last Modified: | 17 Nov 2025 07:13 |
| URI: | http://repository.stfi.ac.id/id/eprint/3465 |
Actions (login required)
 |
View Item |
