ROBIATUL LAILI WILDANI, - (2025) PENGARUH KOMBINASI ZAT PENGATUR TUMBUH NAPHTHALENEACETIC ACID DAN 6- BENZYLAMINOPURIN SERTA ASAM JASMONAT TERHADAP KADAR ANDROGAFOLID PADA KULTUR KALUS SAMBILOTO. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Sambiloto merupakan tanaman obat tradisional yang memiliki aktivitas farmakologi seperti antiinflamasi, antioksidan, antidiabetes. Kandungan bioaktif utama pada tanaman sambiloto yaitu Androgafolid yang termasuk golongan senyawa diterpen lakton. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi zat pengatur tumbuh NAA (Naphthaleneacetic Acid) dan BAP (6- Benzylaminopurin) serta elisitor asam jasmonat (AJ) terhadap kadar andrografolid pada kultur kalus sambiloto (Andrographis paniculata). Kalus diinduksi menggunakan media MS dengan kombinasi NAA dan BAP (1:1, 1:2 dan 2:1), kemudian dielisitasi menggunakan asam jasmonat (AJ) dengan variasi konsentrasi 25 ppm, 50 ppm, 75 ppm dan 100 ppm. Parameter yang diamati meliputi waktu tumbuh kalus, warna, tekstur dan bobot kalus. Kadar andrografolid dianalisis menggunakan instrumen KCKT. Hasil penelitian menunjukkan bahwa kombinasi 2 mg/L NAA dan 1 mg/L BAP menghasilkan kalus paling optimal dengan tekstur kompak dan warna hijau sedikit kecoklatan. Pemberian elisitor asam jasmonat pada konsentrasi 25 ppm memberikan pengaruh terbaik terhadap kadar andrografolid, dengan rata-rata kadar sebesar 71,26% dan nilai AUC tertinggi 1919,9. Peningkatan kadar andrografolid ini diduga terjadi melalui aktivasi jalur pensinyalan jasmonat yang melibatkan ekspresi gen HMGR, DXR, dan GGPP. Penelitian ini menunjukkan bahwa kombinasi ZPT dan elisitor dapat mengoptimalkan produksi metabolit sekunder secara in vitro. ------ Sambiloto is a traditional medicinal plant that has activities as an anticancer, antiinflammatory, antioxidant, antidiabetic. The main bioactive content in the sambiloto plant is Androgafolid which belongs to the diterpene lactone compound group. This study aims to determine the effect of the combination of plant growth regulators NAA (Naphthaleneacetic Acid) and BAP (6-Benzylamino Purin) and jasmonic acid (AJ) elicitor on andrographolide levels in sambiloto (Andrographis paniculata) callus cultures. Callus was induced using MS media with a combination of NAA and BAP (1:1, 1:2 and 2:1), then elicited using jasmonic acid (AJ) with varying concentrations of 25 ppm, 50 ppm, 75 ppm and 100 ppm. The macroscopic parameters observed included callus growth time, color, texture and callus weight. Andrographolide levels were analyzed using a HPLC instrument. The results showed that the combination of 2 mg/L NAA and 1 mg/L BAP produced the most optimal callus with a compact texture and green color. Administration of jasmonic acid elicitor at a concentration of 25 ppm had the best effect on andrographolide levels, with an average level of 71.26% and the highest AUC value of 1919.9. This increase in andrographolide levels is thought to occur through the activation of the jasmonate signaling pathway involving the expression of the HMGR, DXR, and GGPP genes. This study shows that the combination of PGR and elicitor can optimize the production of secondary metabolites in vitro.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Sambiloto, Kalus, NAA, BAP, Asam Jasmonat, Andrografolid. ---- Andrographis paniculata, Callus, NAA, BAP, Jasmonic Acid, Andrographolide. |
| Subjects: | Q Science > QH Natural history > QH301 Biology R Medicine > R Medicine (General) |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 09 Sep 2025 01:56 |
| Last Modified: | 09 Sep 2025 01:57 |
| URI: | http://repository.stfi.ac.id/id/eprint/2772 |
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