PENAPISAN DAN IDENTIFIKASI BAKTERI PENGHASIL BIOSURFAKTAN YANG DIISOLASI DARI Virgin Coconut Oil (VCO)

MUHAMMAD YUSUF DARUSALAM, - (2025) PENAPISAN DAN IDENTIFIKASI BAKTERI PENGHASIL BIOSURFAKTAN YANG DIISOLASI DARI Virgin Coconut Oil (VCO). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Biosurfaktan adalah molekul aktif permukaan yang dihasilkan mikroorganisme untuk menurunkan tegangan permukaan. Penelitian ini bertujuan mengisolasi dan mengidentifikasi bakteri penghasil biosurfaktan dari virgin Coconut Oil (VCO) yang diperoleh melalui fermentasi alami santan kelapa. Isolasi dilakukan dengan pengenceran berseri dan penanaman pada nutrient agar, diikuti karakterisasi makroskopis dan mikroskopis (pewarnaan Gram). Hasil menunjukkan isolat bakteri gram positif berbentuk kokus, diduga Leuconostoc spp., bersifat non-patogen, dan menghasilkan asam laktat. pertumbuhan optimal terjadi pada pengenceran 10-4 dengan 36 koloni. Aktivitas biosurfaktan terkonfirmasi melalui uji emulsifikasi (1,2 cm dan 1,1 cm), oil spreading (zona bening), dan drop collapse (tetesan datar), menandakan kemampuan tinggi menurunkan tegangan permukaan. Penelitian ini membuktikan bahwa VCO berpotensi sebagai sumber bakteri penghasil biosurfaktan alami yang ramah lingkungan. ----- Biosurfactants are surface-active molecules produced by microorganisms to reduce surface tension. This study aimed to isolate and identify biosurfactant-producing bacteria from virgin coconut Oil (VCO) obtained through the natural fermentation of coconut milk. Isolation was carried out using serial dilution and plating on nutrient agar, followed by macroscopic and microscopic characterization (Gram staining). The results showed that the bacterial isolate was gram-positive cocci, presumed to be Leuconostoc spp., non-pathogenic, and capable of producing lactic acid. Optimal growth occurred at the 10-4 dilution with 36 colonies. Biosurfactant activity was confirmed through emulsification tests (1.2 cm and 1.1 cm), oil spreading, and drop collapse, indicating a high ability to reduce surface tension. This study demonstrates that VCO has the potential as a source of natural, environmentally friendly biosurfactant-producing bacteria.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Bakteri, biosurfaktan, isolasi, karakterisasi, virgin coconut oil (VCO). ----- Bacteria, biosurfactant, isolation, characterization, virgin coconut oil (VCO).
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	04 Sep 2025 01:36
Last Modified:	04 Sep 2025 01:36
URI:	http://repository.stfi.ac.id/id/eprint/2753

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