MUHAMAD AD MUARIF, - (2025) PENGARUH ZAT PENGATUR TUMBUH NAA DAN BAP TERHADAP KADAR ANDROGRAFOLID BERDASARKAN WAKTU PANEN PADA KULTUR KALUS SAMBILOTO (Androghapis paniculata (Burm. f.) Ness). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Andrografolid merupakan senyawa aktif utama dalam tanaman sambiloto (Andrographis paniculata) yang memiliki berbagai aktivitas farmakologis, termasuk sebagai antiinflamasi, antibakteri, dan imunostimulan. Upaya peningkatan produksi andrografolid dapat dilakukan melalui teknik kultur jaringan tanaman, khususnya kultur kalus dengan penambahan zat pengatur tumbuh. Penelitian ini bertujuan untuk mengevaluasi pengaruh kombinasi zat pengatur tumbuh (ZPT) Napthaleneacetic Acid (NAA) dan Benzil Amino Purin (BAP) terhadap kadar andrografolid pada kultur kalus tanaman sambiloto (Andrographis paniculata), dengan memperhatikan waktu panen yang optimal pada minggu ke-2. Eksplan daun sambiloto dikulturkan dalam media MS dengan kombinasi konsentrasi NAA dan BAP (1:1, 2:1, 1:2) serta Media MS sebagai kontrol. Pengamatan dilakukan terhadap pertumbuhan kalus, warna, tekstur, bobot basah dan kering, serta kadar andrografolid menggunakan metode KCKT. Hasil menunjukkan bahwa kombinasi 2mg/L NAA : 1mg/L BAP memberikan hasil optimal dalam pembentukan kalus yang cepat, tekstur padat, bobot tinggi, dan kadar andrografolid tertinggi 89.34% Waktu panen terbaik diperoleh pada minggu ke-2 atau 30 Hari Setelah Tanam (HST), di mana kadar andrografolid mencapai puncaknya sebelum mengalami penurunan akibat penuaan kultur. Penelitian ini memberikan kontribusi terhadap optimasi produksi metabolit sekunder melalui teknik kultur jaringan. ------ Andrographolide is the main active compound in Andrographis paniculata (sambiloto) with various pharmacological activities, including anti-inflammatory, antibacterial, and immunostimulant effects. Efforts to increase andrographolide production can be carried out through plant tissue culture techniques, particularly callus culture with the addition of plant growth regulators (PGRs). This study aimed to evaluate the effect of a combination of the PGRs Naphthaleneacetic Acid (NAA) and Benzyl Amino Purine (BAP) on andrographolide content in A. paniculata callus culture, with attention to the optimal harvest time at the 2nd week. Sambiloto leaf explants were cultured on MS medium with NAA and BAP concentration combinations (1:1, 2:1, 1:2) and MS medium as the control. Observations were made on callus growth, color, texture, fresh and dry weight, and andrographolide content using the HPLC method. The results showed that the combination of 2 mg/L NAA : 1 mg/L BAP yielded optimal results in rapid callus formation, firm texture, high biomass, and the highest andrographolide content of 89.34%. The best harvest time was at the 2nd week or 30 Days After Planting (DAP), when andrographolide content peaked before declining due to culture aging. This study contributes to the optimization of secondary metabolite production through tissue culture techniques.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Sambiloto, kultur kalus, NAA, BAP, andrografolid, waktu panen. ---- Andrographis paniculata, callus culture, NAA, BAP, andrographolide, harvest time |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 01 Sep 2025 04:39 |
| Last Modified: | 01 Sep 2025 04:39 |
| URI: | http://repository.stfi.ac.id/id/eprint/2751 |
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