NISA SAONAH, - (2023) PENETAPAN KADAR FENOL TOTAL, AKTIVITAS ANTIOKSIDAN DAN NILAI SUN PROTECTION FACTOR (SPF) PADA EKSTRAK KULIT KOPI ROBUSTA (Coffea canephora L.). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Kulit kopi merupakan produk samping dari proses pengolahan kopi. Kulit kopi mengandung golongan fenol yang memiliki aktivitas antioksidan. Antioksidan dapat menangkal radikal bebas yang terbentuk melalui sinar ultraviolet. Penelitian ini bertujuan mengetahui kadar fenol total, aktivitas antioksidan, dan nilai Sun Protection Factor (SPF) dari ekstrak kulit kopi robusta (Coffea canephora L.) pada berbagai kepolaran pelarut. Ekstraksi dilakukan bertingkat secara sinambung menggunakan Soxhlet dengan pelarut n-heksan, etil asetat, dan etanol 96%. Penetapan kadar fenol total dilakukan dengan metode Folin-Ciocalteu, penetapan aktivitas antioksidan dilakukan dengan metode DPPH dan penetapan nilai SPF dilakukan dengan metode spektrofotometri UV-Vis pada panjang gelombang 290 sampai 320 nm dengan interval 5 nm. Kadar fenol total dinyatakan dalam Gallic Acid Equivalent (GAE) dengan hasil pada ekstrak etanol 96% sebesar 10,963 gGAE/100 g, ekstrak etil asetat sebesar 8,295 gGAE/100 g dan ekstrak n-heksan sebesar 2,452 gGAE/100 g. Berdasarkan nilai IC50, ekstrak etanol 96% (10,582 ppm) antioksidan sangat kuat, ekstrak etil asetat (20,345 ppm) antioksidan sangat kuat, dan ekstrak n-heksan (72,648 bpj) antioksidan kuat. Penetapan nilai SPF dilakukan terhadap semua ekstrak pada konsentrasi 100 hingga 500 bpj. Diperoleh hasil nilai SPF pada konsentrasi 500 bpj, ekstrak etanol 96% 14, pada ekstrak etil asetat 13 dan keduanya termasuk pada proteksi maksimal dan pada ekstrak n heksan 2, termasuk pada proteksi minimal. Dapat disimpulkan ekstrak etanol 96% kulit kopi robusta memiliki kadar fenol total yang paling besar dan paling berpotensi sebagai tabir surya dengan aktivitas antioksidan sangat lemah. --- Coffee skin is a by-product of the coffee processing process. Coffee skin contains phenol groups that have antioxidant activity. Antioxidants can counteract free radicals that are formed through ultraviolet light. This study aims to determine the total phenolic content, antioxidant activity, and Sun Protection Factor (SPF) value of robusta coffee peel extract (Coffea canephora L.) in various solvent polarities. Extraction was carried out in stages continuously using Soxhlet with n-hexane, ethyl acetate and 96% ethanol as solvents. Determination of total phenol content was carried out by the Folin-Ciocalteu method, the antioxidant activity was determined by the DPPH method and the SPF value was determined by the UV-Vis spectrophotometry method at a wavelength of 290 to 320 nm with an interval of 5 nm. Total phenolic content was expressed in Gallic Acid Equivalent (GAE) with a yield of 96% ethanol extract of 10.963 gGAE/100 g, ethyl acetate extract of 8.295 gGAE/100 g and n-hexane extract of 2.452 gGAE/100 g. Based on the IC50 value, 96% ethanol extract (10.582 ppm) was a very strong antioxidant, ethyl acetate extract (20.345 ppm) was a very strong antioxidant, and n-hexane extract (72.648 ppm) was a strong antioxidant. The SPF value was determined for all extracts at a concentration of 100 to 500 ppm. The SPF value was obtained at a concentration of 500 ppm, 96% ethanol extract 14, in ethyl acetate 13 extract and both included in the maximum protection and in the n-hexane 2 extract, including in the minimum protection. It can be concluded that the 96% ethanol extract of robusta coffee peel has the highest total phenol content and has the most potential as a sunscreen with very weak antioxidant activity.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Kulit Kopi, Fenol Total, Antioksidan, Sun Protection Factor. Skin Waste, Total Phenol, Antioxidant, Sun Protection Factor. |
| Subjects: | R Medicine > R Medicine (General) R Medicine > RS Pharmacy and materia medica |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 21 Jun 2024 03:39 |
| Last Modified: | 21 Jun 2024 03:39 |
| URI: | http://repository.stfi.ac.id/id/eprint/275 |
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