MARIA EIRINE KRISNAWATI, - (2025) ISOLASI DAN IDENTIFIKASI BAKTERI ASOSIASI SPONS LAUT Petrosia nigricans YANG BERPOTENSI SEBAGAI ANTIBAKTERI DARI KEPULAUAN SERIBU JAKARTA. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Peningkatan resistensi antibiotik memerlukan upaya eksplorasi sumber senyawa antibakteri baru, salah satunya melalui bakteri asosiasi spons laut Petrosia nigricans. Penelitian ini bertujuan untuk mengisolasi, mengidentifikasi, dan mengevaluasi aktivitas antibakteri bakteri asosiasi spons laut. Sebanyak 15 isolat berhasil diperoleh dengan morfologi sel meliputi Streptococcus, monobasil, kokobasil, dan Staphylococcus. Hasil uji aktivitas antibakteri menunjukkan bahwa isolat 01-PND-7.1 memiliki daya hambat tertinggi terhadap Escherichia coli (18,117 mm), sedangkan isolat 03-PND-5 menunjukkan daya hambat tertinggi terhadap Staphylococcus aureus (7,02 mm). Identifikasi molekuler berdasarkan sekuens gen 16S rRNA mengonfirmasi identitas isolat potensial, diketahui isolat bakteri 01-PND-7.1 memiliki kekerabatan dengan spesies bakteri Bacillus licheniformis yang telah dibuktikan dengan analisis filogenetik. Temuan ini memperlihatkan potensi bakteri asosiasi P. nigricans sebagai kandidat sumber agen antibakteri baru yang berpotensi dikembangkan dalam bidang farmasi. ----- The increasing prevalence of antibiotic resistance necessitates the exploration of novel antibacterial compound sources, one of which is marine sponge-associated bacteria from Petrosia nigricans. This study aimed to isolate, identify, and evaluate the antibacterial activity of sponge-associated bacteria. A total of 15 isolates were obtained, exhibiting cell morphologies including Streptococcus, monobacillus, coccobacillus, and Staphylococcus. Antibacterial activity assays revealed that isolate 01-PND-7.1 exhibited the highest inhibitory activity against Escherichia coli (18.117 mm), while isolate 03-PND-5 showed the highest inhibitory activity against Staphylococcus aureus (7.02 mm). Molecular identification based on 16S rRNA gene sequences confirmed the identity of the potential isolate, with isolate 01-PND7.1 showing close phylogenetic affiliation to Bacillus licheniformis, as supported by phylogenetic analysis. These findings highlight the potential of P. nigricansassociated bacteria as a promising candidate source of novel antibacterial agents for pharmaceutical development.
| Item Type: | Thesis (Skripsi) |
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| Uncontrolled Keywords: | Resistensi antibiotik, Petrosia nigricans, bakteri asosiasi, Bacillus licheniformis, aktivitas antibakteri, identifikasi molekuler. ------ Antibiotic resistance, Petrosia nigricans, associated bacteria, Bacillus licheniformis, antibacterial activity, molecular identification. |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 01 Sep 2025 03:18 |
| Last Modified: | 01 Sep 2025 03:18 |
| URI: | http://repository.stfi.ac.id/id/eprint/2744 |
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