NANDA PUTRI SALSABILA, - (2025) PERBANDINGAN AKTIVITAS INHIBISI TIROSINASE PADA EKSTRAK KULIT BUAH KOPI ARABIKA (Coffea arabica L.) BERDASARKAN PERBEDAAN TEMPAT TUMBUH. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Penelitian ini bertujuan untuk mengidentifikasi pengaruh perbedaan tempat tumbuh kopi Arabika (Coffea arabica L.) terhadap aktivitas inhibisi tirosinase dari ekstrak kulit buah kopi Arabika. Pengujian inhibisi tirosinase bertujuan untuk melihat potensi ekstrak kulit buah kopi Arabika dari tempat tumbuh yang berbeda sebagai agen pencerah kulit. Dua lokasi budidaya kopi Arabika yang dibandingkan adalah daerah Leles, Kabupaten Garut (KLKG) dengan ketinggian 900–1300 mdpl, dan daerah Ibun, Kabupaten Bandung (KIKB) dengan ketinggian 1300–1700 mdpl. Kulit buah kopi Arabika dari masing-masing daerah diekstraksi menggunakan metode pelarut tertentu, kemudian dilakukan uji aktivitas inhibisi tirosinase secara in vitro menggunakan substrat L-DOPA dan pembacaan absorbansi pada panjang gelombang 475 nm dengan alat microplate reader. Nilai IC50 dihitung dari masing-masing sampel dan dianalisis menggunakan uji ANOVA satu arah (One-Way ANOVA) untuk mengetahui apakah terdapat perbedaan yang signifikan secara statistik. Hasil penelitian menunjukkan bahwa terdapat perbedaan yang signifikan (p < 0,05) antara nilai IC50 dari ekstrak kulit buah kopi Arabika asal Leles dan Ibun. Dapat disimpulkan bahwa perbedaan tempat tumbuh yang ditandai dengan perbedaan ketinggian berpengaruh nyata terhadap aktivitas inhibisi tirosinase dari ekstrak kulit buah kopi Arabika. ----- This study aims to identify the effect of different growing places of Arabica coffee (Coffea arabica L.) on the tyrosinase enzyme inhibition activity of Arabica coffee fruit skin extract. Tyrosinase inhibition testing aims to see the potential of Arabica coffee fruit skin extracts from different growing places as a skin whitening agent. The two Arabica coffee cultivation sites compared were Leles, Garut Regency (KLKG) at 900-1300 meters above sea level, and Ibun, Bandung Regency (KIKB) at 1300-1700 meters above sea level. The Arabica coffee fruit skin from each region was extracted using a specific solvent method, then tested for tyrosinase inhibition activity in vitro using L-DOPA substrate and absorbance reading at a wavelength of 475 nm with a microplate reader. The IC50 value was calculated from each sample and analyzed using the One-Way ANOVA test to determine whether there were statistically significant differences. The results showed that there was a significant difference (p < 0.05) between the IC50 values of Arabica coffee fruit skin extracts from Leles and Ibun. It can be concluded that differences in the place of growth characterized by differences in altitude significantly affect the tyrosinase inhibition activity of Arabica coffee fruit peel extract.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Ekstrak Kulit buah kopi arabika (Coffea arabica L), Leles, Ibun, tirosinase. ------ Arabica coffee fruit skin extract (Coffea arabica L), Leles, Ibun, tyrosinase. |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 28 Aug 2025 07:26 |
| Last Modified: | 28 Aug 2025 07:26 |
| URI: | http://repository.stfi.ac.id/id/eprint/2710 |
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