OPTIMALISASI PARAMETER EKSTRAKSI DAN APLIKASI FLAVOR MASKING UNTUK PENINGKATAN ACCEPTABILITY SENSORY DAUN STEVIA (Stevia Rebaudiana Bert)

JOÃO BOSCO SOARES PINTO, - (2025) OPTIMALISASI PARAMETER EKSTRAKSI DAN APLIKASI FLAVOR MASKING UNTUK PENINGKATAN ACCEPTABILITY SENSORY DAUN STEVIA (Stevia Rebaudiana Bert). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Stevia (Stevia rebaudiana Bert) merupakan pemanis alami non-kalori dengan tingkat kemanisan 200–300 kali lebih tinggi dari gula tebu. Namun, penggunaan stevia sering terkendala oleh aftertaste pahit serta rasa/aroma langu akibat kandungan tanin, flavonoid, dan 3-methyl-butanal. Penelitian ini bertujuan mengurangi aftertaste pahit dan rasa/aroma langu tanpa menurunkan rasa manis stevia, melalui optimalisasi ekstraksi suhu rendah dan penambahan sinamaldehida sebagai flavor masking. Ekstraksi dilakukan dengan metode maserasi pada suhu -18°C selama 60 menit menggunakan pelarut metanol dan etanol dengan rasio 1:5 dan 1:10, kemudian ditambahkan sinamaldehida 0,2%. Evaluasi meliputi uji skrining fitokimia, loss on drying (LOD), uji hedonik (40 panelis), dan analisis residu metanol dengan GC-MS. Hasil menunjukkan ekstraksi menggunakan metanol rasio 1:10 menghasilkan rendemen tertinggi (3,00%) dengan kandungan tanin dan flavonoid positif. Perlakuan dengan metanol 1:10 dan penambahan sinamaldehida memberikan skor hedonik terbaik: rasa manis 7,15, rasa pahit 3,25, dan rasa/aroma langu 2,35. Analisis GC-MS memastikan tidak adanya residu metanol. Dengan demikian, kombinasi ekstraksi suhu rendah dan sinamaldehida terbukti efektif mengurangi aftertaste pahit dan rasa/aroma langu stevia tanpa mengurangi rasa manisnya. ------ Stevia (Stevia rebaudiana Bert) is a natural non-caloric sweetener with sweetness 200–300 times higher than cane sugar. However, its use is limited by a bitter aftertaste and undesirable grassy taste/aroma caused by tannins, flavonoids, and 3-methyl-butanal. This study aimed to reduce the bitter aftertaste and grassy notes without diminishing sweetness through low-temperature extraction and the application of cinnamaldehyde as a flavor-masking agent. The extraction was performed by maceration at -18°C for 60 minutes using methanol and ethanol solvents at 1:5 and 1:10 ratios, followed by the addition of 0.2% cinnamaldehyde. The evaluation included phytochemical screening, loss on drying (LOD), hedonic testing with 40 panelists, and methanol residue analysis by GC-MS. The results showed that methanol extraction at a 1:10 ratio yielded the highest extract (3.00%) with positive tannin and flavonoid content. The methanol 1:10 treatment with cinnamaldehyde addition gave the best hedonic scores: highest sweetness (7.15), lowest bitterness (3.25), and lowest grassy taste/aroma (2.35). GC-MS analysis confirmed the absence of methanol residue in the final sample. In conclusion, combining low-temperature extraction and cinnamaldehyde effectively minimized the bitter aftertaste and grassy notes of stevia without reducing its sweetness.

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Stevia, pemanis alami, ekstraksi suhu rendah, flavor masking. ---- Stevia, natural sweetener, low-temperature extraction, flavor masking.
Subjects: R Medicine > R Medicine (General)
R Medicine > RS Pharmacy and materia medica
Divisions: Program Studi S1 Farmasi
Depositing User: pustakawan - -
Date Deposited: 28 Aug 2025 02:35
Last Modified: 28 Aug 2025 02:35
URI: http://repository.stfi.ac.id/id/eprint/2706

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