ELIZABETH TANIA APRIAN, - (2025) AKTIVITAS INHIBISI TIROSINASE DARI EKSTRAK KULIT BUAH KOPI ROBUSTA (Coffea canephora L.). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Penelitian ini bertujuan mengevaluasi aktivitas inhibisi enzim tirosinase dari berbagai fraksi ekstrak kulit buah kopi robusta (Coffea canephora L.) dan senyawa bioaktif di dalamnya. Sampel yang diuji meliputi ekstrak etanol 96%, etil asetat, nheksana, asam kafeat, dan kafein, dengan asam kojat sebagai kontrol positif. Uji inhibisi dilakukan melalui pengukuran % inhibisi dan nilai IC₅₀. Hasil menunjukkan bahwa fraksi n-heksana memiliki potensi tertinggi (IC₅₀ = 77,117 µg/mL), diikuti etanol 96% dan etil asetat. Uji ANOVA mengonfirmasi tidak adanya perbedaan signifikan antar kelompok sampel uji. Hasil ini menunjukkan bahwa aktivitas penghambatan tirosinase berasal dari sinergi berbagai senyawa bioaktif, terutama yang non-polar. Penelitian lanjutan disarankan untuk mengidentifikasi senyawa aktif utama, mengkaji mekanisme inhibisi, serta mengembangkan formulasi kosmetik berbasis ekstrak kulit buah kopi robusta. ----- This study aims to evaluate the tyrosinase inhibitory activity of various fractions of robusta coffee (Coffea canephora L.) fruit peel extract and its bioactive compounds. The samples tested included 96% ethanol extract, ethyl acetate, n-hexane, caffeic acid, and caffeine, with kojic acid as a positive control. The inhibition test was carried out by measuring the % inhibition and IC₅₀ values. The results showed that the n-hexane fraction had the highest potency (IC₅₀ = 77.117 µg/mL), followed by 96% ethanol and ethyl acetate. ANOVA test confirmed no significant differences between the test sample groups. These results indicate that the tyrosinase inhibitory activity comes from the synergy of various bioactive compounds, especially nonpolar ones. Further research is recommended to identify the main active compounds, examine the inhibition mechanism, and develop cosmetic formulations based on robusta coffee fruit peel extract.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Kulit buah kopi robusta, Tirosinase, Asam Kafeat, Kafein, Asam Kojat. ----- Robusta coffee fruit skin, tyrosinase, IC₅₀, n-hexane, caffeic acid, caffeine, kojic acid. |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 13 Aug 2025 04:27 |
| Last Modified: | 28 Aug 2025 01:58 |
| URI: | http://repository.stfi.ac.id/id/eprint/2699 |
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