VALIDASI METODE ANALISIS KADAR METHEMOGLOBIN DALAM DARAH MENGGUNAKAN SPEKTROFOTOMETRI UV-VISIBEL

NADHILA, - (2023) VALIDASI METODE ANALISIS KADAR METHEMOGLOBIN DALAM DARAH MENGGUNAKAN SPEKTROFOTOMETRI UV-VISIBEL. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Pengukuran kadar methemoglobin (metHb) dalam darah menjadi penting untuk penapisan dan penegakan diagnosis kondisi metemoglobinemia. Pengukuran kadar methemoglobin dengan menggunakan NaCN merupakan alternatif karena pertimbangan ketersediaan dan optimalisasi biaya. Tujuan dari penelitian ini adalah untuk mengevaluasi kinerja metode analisis kadar methemoglobin dalam darah menggunakan spektrofotometri UV-Visibel dengan reagen NaCN. Metode ini dievaluasi berdasarkan parameter linearitas, akurasi, presisi, batas deteksi (LOD), batas kuantifikasi (LOQ). Hasil validasi menunjukkan bahwa metode spektrofotometri UV�Visibel dengan reagen NaCN memiliki rentang linearitas antara 1,1% hingga 11,4% metHb dengan nilai r = 0,9862; uji akurasi dengan rata-rata perolehan kembali berada pada rentang 100 - 101,884%; dan presisi inter-day dievaluasi dengan koefisien variasi7,01%. LOD dan LOQ masing-masing dihitung sebesar 1,84% dan 5,92% metHb. Berdasarkan hasil penelitian dapat disimpulkan bahwa spektrofotometri uv-vis pada analisis kadar methemoglobin menggunakan reagen NaCN tervalidasi atau valid karena terpenuhinya kriteria dari parameter validasi. --- Measurement of methemoglobin (metHb) levels in the blood is important for the diagnosis and monitoring of methemoglobinemia conditions. The analytical method using UV-Visible spectrophotometry with NaCN is an alternative because NaCN has economic value and is easy to obtain. The aim of this study was to validate the UV-Visible spectrophotometric analysis method with NaCN reagent for measuring metHb levels in blood samples. This method is evaluated based on the parameters of linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ). The validation results showed that the UV-Visible spectrophotometry method with NaCN reagent has a linearity range between 1.1% to 11.4% metHb with a value of r = 0.9862; accuracy test with average recovery in the range of 100 - 101.884%; and the inter-day precision was evaluated with a coefficient of variation of 7.01%. LOD and LOQ were calculated at 1.84% and 5.92% metHb, respectively. Based on the results of the study it can be concluded that the uv-vis spectrophotometry in the analysis of methemoglobin levels using the NaCN reagent ist validated because the criteria for the validation parameters are completed.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	validasi metode, methemoglobin, NaCN validation methode, methemoglobin, NaCN
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	15 Jun 2024 04:04
Last Modified:	26 Jun 2024 01:23
URI:	http://repository.stfi.ac.id/id/eprint/227

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