KARAKTERISASI PRODUK BIOSURFAKTAN PADA BAKTERI Staphylococcus epidermidis MENGGUNAKAN METODE FTIR

MARSYA AUDHIA SUWARDI, - (2023) KARAKTERISASI PRODUK BIOSURFAKTAN PADA BAKTERI Staphylococcus epidermidis MENGGUNAKAN METODE FTIR. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Biosurfaktan merupakan molekul ampifilik yang memiliki kemampuan mereduksi tegangan permukaan yang dapat digunakan di lingkungan, industri, pertanian, aktivitas terapeutik dan juga dapat meningkatkan bioremediasi minyak bumi. Bakteri penghasil biosurfaktan salah satunya Staphylococcus epidermidis, yang belum banyak data ditemukan tentang jenis biosurfaktan yang dihasilkan bakteri tersebut. Penelitian ini bertujuan mengkarakteristik produk biosurfaktan yang dihasilkan dari bakteri Staphylococcus epidermidis menggunakan metode Fourier Transform Infrared (FTIR). Produksi biosurfaktan dilakukan pada media Mineral salt medium (MSM) yang ditambahkan minyak jelantah 4% dan pH 7. Biosurfaktan yang dihasilkan dianalisis indeks emulsifikasinya dan dikarakteristik menggunakan FTIR. Indeks Emulsifikasi yang diperoleh sebesar 51% dan hasil karakterisasi menggunakan FTIR menunjukan adanya gugus C=C, CH₂, S-H,gugus karboksilat. Biosurfaktan yang dihasilkan Staphylococcus epidermidis menunjukan golongan lipopeptida. --- Biosurfactants are amphiphilic molecules that have the ability to reduce surface tension that can be used in environmental, industrial, agricultural, therapeutic activities and can also enhance petroleum bioremediation. Biosurfactant-producing bacteria, one of which is Staphylococcus epidermidis, for which has not found much data on the type of biosurfactant that produces by these bacteria. This study aims to characterize biosurfactant products produced from Staphylococcus epidermidis using the Fourier Transform Infrared (FTIR) method. Biosurfactant production was carried out on Mineral salt medium (MSM) which was added with 4% used cooking oil and pH 7. The resulting biosurfactant was analyzed for its emulsification index and characterized using FTIR. The Emulsification Index obtained was 51% and the results of characterization using FTIR showed the presence of C=C, CH₂, SH, carboxylic groups. The biosurfactant produced by Staphylococcus epidermidis shows the lipopeptide class.

Item Type: Thesis (Skripsi)
Uncontrolled Keywords: Biosurfaktan, Staphylococcus epidermidis, MSM, FTIR Biosurfactants, Staphylococcus epidermidis, MSM, FTIR
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Divisions: Program Studi S1 Farmasi
Depositing User: pustakawan - -
Date Deposited: 07 Jun 2024 06:14
Last Modified: 07 Jun 2024 06:14
URI: http://repository.stfi.ac.id/id/eprint/184

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