EVELYN OKTAVANY ISMAIL, - (2024) MEKANISME KEMATIAN SEL KANKER PROSTAT DU-145 PADA PEMBERIAN ISOLAT ANDROGRAFOLID MELALUI JALUR INTRINSIK DENGAN MENGGUNAKAN METODE IN CELL WESTERN SECARA IN VITRO. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Indonesia merupakan negara dengan keberagaman tumbuhan yang melimpah, salah satunya adalah Andrographis paniculata Nees (A. paniculata) atau sambiloto, umumnya dikenal sebagai ‘King of Bitter’, merupakan tanaman kecil, tahunan, dan bercabang, yang tergolong ke dalam keluarga Acanthaceae. Tanaman ini digunakan secara tradisional untuk berbagai penyakit, dan senyawa utamanya, andrografolid, menunjukkan potensi sebagai agen antikanker. Kanker prostat, dengan angka kejadian tinggi, menjadi fokus penting dalam penelitian ini. Terapi kanker konvensional sering menimbulkan efek samping, mendorong pencarian alternatif berbasis tanaman. Penelitian sebelumnya mengindikasikan bahwa isolat andrografolid dapat menghambat pertumbuhan sel kanker dengan nilai IC50 49,35 µg/mL. Penelitian ini bertujuan untuk mengeksplorasi mekanisme kematian sel kanker prostat DU-145 oleh isolat andrografolid melalui jalur intrinsik apoptosis dengan metode In-Cell Western. Hasil menunjukkan isolat andrografolid menginduksi kematian sel melalui aktivasi caspase-3, dengan nilai IC50 rata-rata 107,31 µg/mL. Temuan ini mendukung potensi isolat andrografolid sebagai alternatif terapi kanker prostat yang lebih efektif dibandingkan kemoterapi konvensional. Penelitian ini berkontribusi penting terhadap pemahaman mekanisme kerja tanaman obat dalam pengobatan kanker. ----- Indonesia is a country with an abundant diversity of plants, one of which is Andrographis paniculata Nees (A. paniculata) or bitter, commonly known as 'King of Bitter', which is a small, annual and branching plant, which belongs to the Acanthaceae family. This plant is used traditionally for various diseases, and its main compound, andrographolide, shows potential as an anticancer agent. Prostate cancer, with its high incidence, was an important focus in this study. Conventional cancer therapies often cause side effects, prompting the search for plant-based alternatives. Previous research indicated that andrographolide isolates could inhibit the growth of cancer cells with an IC50 value of 49.35 µg/mL. This study aims to explore the mechanism of death of DU-145 prostate cancer cells by andrographolide isolates through the intrinsic pathways of apoptosis using the In-Cell Western method. The results showed that the andrographolide isolates induced cell death through activation of caspase-3, with an average IC50 value of 107.31 µg/mL. These findings support the potential of andrographolide isolates as an alternative prostate cancer therapy that is more effective than conventional chemotherapy. This research makes an important contribution to understanding the mechanism of action of medicinal plants in cancer treatment.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Andrographis, Sambiloto, Kanker Prostat, DU-145, In-Cell Western, Caspase 3, IC50. ----- Andrographis, Sambiloto, Prostate Cancer, DU-145, In-Cell Western, Caspase 3, IC50. |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 12 Nov 2024 09:14 |
| Last Modified: | 12 Nov 2024 09:14 |
| URI: | http://repository.stfi.ac.id/id/eprint/1474 |
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