UJI TOKSISITAS TERATOGENIK DAN PROFIL HISTOPATOLOGI TERHADAP PENGGUNAAN ISOLAT ANDROGRAFOLID PADA MENCIT SWISS WEBSTER BETINA

AULIYA USWATURROBBANI, - (2024) UJI TOKSISITAS TERATOGENIK DAN PROFIL HISTOPATOLOGI TERHADAP PENGGUNAAN ISOLAT ANDROGRAFOLID PADA MENCIT SWISS WEBSTER BETINA. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

	Text S_PSFF_A201069_Title.pdf Download (380kB)
	Text S_PSFF_A201069_Chapter1.pdf Restricted to Repository staff only Download (37kB)
	Text S_PSFF_A201069_Chapter2.pdf Restricted to Repository staff only Download (161kB)
	Text S_PSFF_A201069_Chapter3.pdf Restricted to Repository staff only Download (106kB)
	Text S_PSFF_A201069_Chapter4.pdf Restricted to Repository staff only Download (261kB)
	Text S_PSFF_A201069_Chapter5.pdf Restricted to Repository staff only Download (29kB)
	Text S_PSFF_A201069_Appendix.pdf Restricted to Repository staff only Download (475kB)

Abstract

Andrografolid merupakan senyawa yang paling dominan dari tanaman sambiloto. Penelitian yang sangat komprehensif terhadap andrografolid sudah dilakukan pada tikus dan kelinci. Hasil penelitian tersebut menunjukkan bahwa sambiloto menyebabkan aborsi selama kebuntingan, diduga karena efeknya yang berlawanan dengan hormon progesteron endogen. Uji teratogenik dilakukan untuk mengevaluasi efek pemberian obat selama masa organogenesis. Hewan percobaan diaklimatisasi selama 1 minggu sebelum pengujian. Mencit betina dalam fase estrus ditempatkan bersama mencit jantan dalam satu kandang untuk pembuahan, lalu dipisahkan setelah pembuahan yang ditandai dengan adanya sperma untuk menentukan hari pertama kebuntingan. Semua mencit ditimbang secara berkala. Kelompok uji diberikan isolat andrografolid secara oral dengan dosis 100, 500, dan 1000 mg/kgBB selama kebuntingan pada hari ke-6 sampai hari ke-15. Pada hari ke18 kebuntingan, induk dibedah dan dilakukan evaluasi makroskopik serta pemeriksaan fetus. Pemeriksaan fetus meliputi morfologi anggota tubuh, tulang serta jaringan lunak. Hasil penelitian menunjukkan bahwa pemberian isolat andrografolid selama masa organogenesis memiliki efek toksik pada fetus, yang menyebabkan abnormalitas seperti hemoragi, tubuh kerdil, bongkok, open eyelids, serta telinga yang belum terbentuk sempurna dan gangguan perkembangan tulang belakang. Selain itu, pengamatan histopatologi hati mencit menunjukkan kerusakan sel hati. Oleh karena itu, penggunaan isolat andrografolid perlu dilakukan dengan hati-hati terutama pada ibu hamil. ----- Andrographolide is the most dominant compound from the bitter plant. Very comprehensive research on andrographolide has been carried out on mice and rabbits. The results of this study showed that bitter caused abortion during pregnancy, allegedly because of its opposing effect on the endogenous hormone progesterone. Teratogenic tests are carried out to evaluate the effects of drug administration during organogenesis. Experimental animals were acclimatized for 1 week before testing. Female mice in the estrous phase are placed with male mice in the same cage for fertilization, then separated after fertilization is marked by the presence of sperm to determine the first day of pregnancy. All mice were weighed periodically. The test group was given andrographolide isolate orally at doses of 100, 500, and 1000 mg/kgBW during pregnancy on days 6 to 15. On the 18th day of pregnancy, the mother is dissected and a macroscopic evaluation and fetal examination is carried out. Fetal examination includes the morphology of body parts, bones and soft tissue. The research results showed that administration of andrographolide isolate during the organogenesis period had a toxic effect on the fetus, which caused abnormalities such as hemorrhage, stunted body, hunchback, open eyelids, as well as ears that were not fully formed and impaired spinal development. In addition, histopathological observations of mice livers showed liver cell damage. Therefore, the use of andrographolide isolate needs to be done with caution, especially in pregnant women.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	andrografolid, histopatologi, OECD 421, sambiloto, teratogenisitas. ---- andrographolide, histopathology, OECD 421, sambiloto, teratogenicity
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	12 Nov 2024 08:25
Last Modified:	12 Nov 2024 08:25
URI:	http://repository.stfi.ac.id/id/eprint/1468

Actions (login required)

View Item