NINDIE AYU, - (2020) PENGARUH PENAMBAHAN ZAT ELISITOR ASAM SALISILAT DAN KITOSAN TERHADAP KANDUNGAN FLAVONOID PADA TANAMAN TIN (Ficus carica L.). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Tanaman tin (Ficus carica L.) mengandung berbagai macam metabolit sekunder, salah satunya flavonoid yang memiliki aktivitas antioksidan cukup tinggi. Flavonoid yang terkandung dalam tanaman tin (Ficus carica L.) dapat ditingkatkan dengan penambahan elisitor. Tujuan dari penelitian ini untuk mengetahui pengaruh penambahan elisitor asam salisilat dan kitosan terhadap peningkatan kadar flavonoid dalam kultur tanaman tin (Ficus carica L.). Multiplikasi tanaman tin (Ficus carica L.) menggunakan media Murashige and Skoog (MS) dan Zat Pengatur Tumbuh (ZPT) yaitu BAP (Benzyl Amino Purin) dan NAA (Naphthalena Acetid Acid). Penambahan elisitor pada planlet dilakukan pada minggu ke-4 dengan konsentrasi asam salisilat yang digunakan yaitu 0,05mM, 0,06mM dan konsentrasi kitosan yang digunakan yaitu 0,2mg/mL, 0,3mg/mL. Planlet diinkubasi kembali selama 48 jam dan dimaserasi menggunakan pelarut etanol 96% selama 3x24 jam. Penetapan kadar flavonoid dilakukan dengan menggunakan spektrofotometer Uv-Vis. Skrinning fitokimia menunjukkan adanya warna merah pada lapisan amil alkohol yang menandakan positif adanya flavonoid. Kadar flavonoid yang didapatkan dari penambahan elisitor dengan konsentrasi asam salisilat 0,05mM 0,760 (gQE/100g ekstrak) dan 0,06mM sebesar 0,459 (gQE/100g ekstrak), sedangkan dari kitosan 0,2mg/mL sebesar 0,980 (gQE/100g ekstrak) dan 0,3mg/mL sebesar 0,720 (gQE/100g ekstrak). Dapat disimpulkan bahwa konsentrasi yang optimal untuk menaikkan kadar flavonoid yaitu kitosan 0,2mg/mL dengan masa inkubasi 48 jam. --- Tin plant (Ficus carica L.) contains various kinds of secondary metabolites, one of which is flavonoids which have high antioxidant activity. The flavonoids contained in the tin plant (Ficus carica L.) can be increased by adding an elicitor. The purpose of this study was to determine the effect of adding salicylic acid and chitosan elicitors to increasing levels of flavonoids in tin plants (Ficus carica L.). The multiplication of tin (Ficus carica L.) using Murashige and Skoog (MS) media and Growth Regulating Substances (ZPT), namely BAP (Benzyl Amino Purin) and NAA (Naphthalena Acetid Acid). The addition of elicitor to the plantlet was carried out at week 4 with the concentration of salicylic acid used, namely 0.05mM, 0.06mM and the precipitation chitosan used was 0.2mg / mL, 0.3mg / mL. The plantlets were incubated again for 48 hours and then macerated using 96% ethanol for 3x24 hours. Determination of flavonoid levels was carried out using a Uv-Vis spectrophotometer. Phytochemical screening showed a red color on the amyl alcohol layer, which indicates a positive presence of flavonoids. The flavonoid results obtained from the addition of an elicitor with a salicylic acid concentration of 0.05mM 0.760 (gQE / 100g extract) and 0.06mM were 0.459 (gQE / 100g extract), while from chitosan 0.2mg / mL was 0.980 (gQE / 100g extract) and 0.3mg / mL of 0.720 (gQE / 100g extract). It can be concluded that the optimal concentration to increase flavonoid levels is chitosan 0.2mg / mL with an incubation period of 48 hours.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Kultur jaringan, Tanaman tin (Ficus carica L.), Flavonoid, Elisitor, Spektrofotometer Uv-Vis. --- Tissue culture, Tin plant (Ficus carica L.), Flavonoid, Elicitor, Spectrophotometer Uv-Vis |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 03 Oct 2024 06:38 |
| Last Modified: | 03 Oct 2024 06:38 |
| URI: | http://repository.stfi.ac.id/id/eprint/1333 |
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