STUDI IN SILICO PERBANDINGAN EPITOP DAN PEMODELAN STRUKTUR ANTIGEN PERTUSSIS TOXIN WILD-TYPE DAN GENETICALLY DETOXIFIED PERTUSSIS TOXIN PADA Bordetella pertussis

NUR MAULIDA AZIZAH, - (2021) STUDI IN SILICO PERBANDINGAN EPITOP DAN PEMODELAN STRUKTUR ANTIGEN PERTUSSIS TOXIN WILD-TYPE DAN GENETICALLY DETOXIFIED PERTUSSIS TOXIN PADA Bordetella pertussis. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Pertussis toxin merupakan racun yang dihasilkan dari bakteri Bordetella pertussis dan dapat menyebabkan penyakit pertusis atau whooping cough pada manusia. Penelitian ini bertujuan untuk memperoleh epitop dari pertussis toxin wild-type dan genetically detoxified pertussis toxin yang akan berikatan dengan antibodi. Data pada penelitian ini diperoleh dengan menggunakan perangkat keras komputer dan perangkat lunak server NCBI, server IEDB (Kolaskar dan Tangoankar, Emini surface accessibility, Karplus dan Schulz's) server Phyre2 dan server ElliPro. Berdasarkan hasil pengolahan data diketahui bahwa terdapat perbedaan pada asam amino pertussis toxin wild-type dan genetically detoxified pertussis toxin. Nilai maksimum aksesibilitas dan fleksibilitas untuk pertussis toxin wild-type 4,120 dan 4,266 untuk genetically detoxified pertussis toxin dengan tingkat kepercayaan homology modelling adalah 100%. Selain itu, untuk prediksi epitop berbasis struktur terdapat 4 epitop inear dan 2 epitop discontinuous pertussis toxin wild-type dan genetically detoxified pertussis toxin dengan skor diatas 0,7. Dengan demikian dapat disimpulkan bahwa epitop yang dihasilkan memiliki jumlah yang sama baik pada pertussis toxin wild-type maupun genetically detoxified pertussis toxin. --- Pertussis toxin is a poison produced by the bacterium Bordetella pertussis and can cause pertussis or whooping cough in humans. This study aims to obtain epitopes of wild-type pertussis toxin and genetically detoxified pertussis toxin that will bind to antibodies. The data in this susly were obtained using computer hardware and NCBI server software, IEDB servers (Kolaskar and Tangoankar, Emini surface accessibility, Karplus and Schultz's) Pkyre2 servers and ElliPro servers. Based on the results of data processing, it is known that there are differences in the amino acids of wild-type pertussis toxin and genetically detoxified pertussis toxin. The maximum value of accessibility and flexibility for wild-type pertussis toxin was 4,120 and 4,266 for genetically detoxified pertussis toxin with a confidence level of 100% homology modeling. In addition, for the structure-based epitope prediction, there are 4 linear epitopes and 2 epitopes of wild-type discontinuous pertussis toxin and genetically detoxified pertussis toxin with scores above 0.7. Thus it can be concluded that the resulting epitope has the same amount of both wild-type pertussis toxin and genetically detoxified pertussis toxin

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	pertussis toxin, epitop, pertussis toxin wild-type, genetically detoxified pertussis toxin --- pertussis toxin, epitop, pertussis toxin wild-type, genetically detoxified pertussis toxin
Subjects:	Q Science > Q Science (General)
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	07 Sep 2024 01:55
Last Modified:	07 Sep 2024 01:55
URI:	http://repository.stfi.ac.id/id/eprint/1115

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