UJI TOKSISITAS AKUT ORAL SENYAWA KOMBINASI KURKUMIN, BRAZILIN DAN MANGOSTIN PADA TIKUS DENGAN METODE STANDAR OECD 425

ANISYA PUTRI SASKIA, - (2024) UJI TOKSISITAS AKUT ORAL SENYAWA KOMBINASI KURKUMIN, BRAZILIN DAN MANGOSTIN PADA TIKUS DENGAN METODE STANDAR OECD 425. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Kandidat suplemen kesehatan dengan formula regimen zat aktif kurkumin, brazilin dan mangostin memiliki potensi aktivitas farmakologi antibakteri, antioksidan dan antiinfalamsi, namun kajian keamannanya belum dilakukan. Penelitian ini bertujuan untuk menguji toksisitas akut oral dari kombinasi isolat kurkumin, mangostin dan brazilin pada tikus menggunakan standar OECD 425. Uji dilakukan pada tikus betina galur wistar dengan berat badan 200-250 gram. dosisi tunggal 5000 mg/kgBB di Berikan secara oral kemudian tikus diamati tanda dan gejala toksisitas selama 14 hari terutama 48 jam pertama dengan pengamatan khusus pada 4 jam pertama setiap 30 menit. Parameter toksisitas yang diamati meliputi gejala toksisitas, perubahan berat badan, adanya kematian serta pengamatan histopatologi. Nilai LD50 >5000 mg/kgBB nilai ini termasuk dalam kategori praktis tidak toksik. Hasil histopatologi menunjukkan adanya perubahan ringan pada organ hati, ginjal, lambung dan limpa. Tingkat toksisitas kandidat suplemen kesehatan dengan formula regimen zat aktif isolat kurkumin, brazilin dan mangostin adalah praktis tidak toksik dengan Nilai LD50 diperoleh >5000 mg/kgBB, Meskipun demikian, adanya perubahan histopatologi ringan menunjukkan perlunya kehati-hatian dalam penggunaan dosis tinggi. ------ The health supplement candidate with the formula regimen for the active substances curcumin, brazilin and mangostin has potential antibacterial, antioxidant and anti-inflammatory pharmacological activity, but safety studies have not been carried out. This study aims to test the acute oral toxicity of the combination of curcumin, brazilin and mangostin isolates in mice using the OECD 425 standard. The test was carried out on female Wistar mice with a body weight of 200-250 grams. A single dose of 5000 mg/kgBW was given orally. Then the mice were observed for signs and symptoms of toxicity for 14 days, especially the first 48 hours with special observations in the first 4 hours every 30 minutes. Toxicity parameters observed included toxicity symptoms, changes in body weight, death and histopathological observations. The LD50 value is >5000 mg/kgBB. This value is included in the practically non-toxic category. Histopathology results showed mild changes in the liver, kidney, stomach and spleen. The level of toxicity of the health supplement candidate with the active ingredient regimen formula of curcumin, brazilin and mangostin isolates is practically non-toxic with an LD50 value obtained >5000 mg/kgBW. However, the presence of mild histopathological changes indicates the need for caution in using high doses.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Kurkumin, Mangostin, Brazilin, Kombinasi, Toksisitas. ------ Curcumin, Mangostin, Brazilin, Combination, Toxicity.
Subjects:	Q Science > Q Science (General) R Medicine > RM Therapeutics. Pharmacology
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	28 Aug 2024 06:28
Last Modified:	28 Aug 2024 06:28
URI:	http://repository.stfi.ac.id/id/eprint/1100

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