DERIVATISASI KATEKIN MELALUI REAKSI ETERIFIKASI DENGAN REAGEN DIMETIL SULFAT

NIDA NIRVANA, - (2024) DERIVATISASI KATEKIN MELALUI REAKSI ETERIFIKASI DENGAN REAGEN DIMETIL SULFAT. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Katekin (C15H14O6, 290 g/mol) adalah senyawa dengan dua cincin aromatik dan lima gugus hidroksil yang memiliki aktivitas antioksidan dan antibakteri, namun aktivitas antioksidannya masih belum optimal. Penelitian ini bertujuan meningkatkan aktivitas antioksidan dan antibakteri katekin melalui derivatisasi, dengan mengubah gugus hidroksil (-OH) menjadi gugus metoksi (-OCH3) menggunakan reagen dimetil sulfat. Struktur produk derivat dielusidasi dan diuji aktivitas antioksidan serta antibakterinya. Hasil penelitian menunjukkan keberhasilan modifikasi katekin, dibuktikan dengan pergeseran panjang gelombang menjadi 278 nm pada spektrum UV-Visibel dan munculnya puncak baru pada bilangan gelombang 2845,79 cm-1 dalam spektrum FTIR, yang mengindikasikan keberadaan gugus metoksi. Pengujian aktivitas antioksidan menunjukkan peningkatan signifikan, dengan nilai IC50 produk derivatisasi sebesar 29 μg/mL, lebih baik dibandingkan isolat katekin awal yang memiliki IC50 76 μg/mL. Aktivitas antibakteri juga menunjukkan peningkatan, dimana pada konsentrasi 100 ppm, isolat katekin menghasilkan luas area hambat 19,38 dan 19,63 mm (kategori sedang), sementara produk derivat menghasilkan zona hambat 21,56 dan 21 mm (kategori kuat). Kesimpulannya, turunan katekin yang dimodifikasi berhasil disintesis dengan aktivitas antioksidan dan antibakteri yang lebih baik dibandingkan senyawa asalnya. ------- Catechin (C15H14O6, 290 g/mol) is a compound with two aromatic rings and five hydroxyl groups that has antioxidant and antibacterial activities, but its antioxidant activity is still not optimal. This study aims to improve the antioxidant and antibacterial activity of catechins through derivatisation, by converting the hydroxyl group (-OH) into a methoxy group (-OCH3) using dimethyl sulphate reagent. The structures of the derivatised products were elucidated and tested for antioxidant and antibacterial activities. The results showed successful modification of catechins, evidenced by a wavelength shift to 278 nm in the UV-Visible spectrum and the appearance of a new peak at wave number 2845.79 cm-1 in the FTIR spectrum, indicating the presence of methoxy groups. Antioxidant activity testing showed significant improvement, with the IC50 value of the derivatised product at 29 μg/mL, better than the initial catechin isolate which had an IC50 of 76 μg/mL. Antibacterial activity also showed improvement, where at a concentration of 100 ppm, the catechin isolate produced inhibition areas of 19.38 and 19.63 mm (medium category), while the derivatised product produced inhibition zones of 21.56 and 21 mm (strong category). In conclusion, modified catechin derivatives were successfully synthesised with better antioxidant and antibacterial activities than the original compound.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Katekin, Derivatisasi, Dimetil Sulfat, Antioksidan, Antibakteri. ------- Catechin, Derivatisation, Dimethyl Sulfate, Antioxidant, Antibacterial
Subjects:	Q Science > Q Science (General) Q Science > QD Chemistry
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	28 Aug 2024 06:14
Last Modified:	28 Aug 2024 06:14
URI:	http://repository.stfi.ac.id/id/eprint/1099

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