DERIVATISASI KUERSETIN DENGAN BENZIL KLORIDA MELALUI REAKSI ETERIFIKASI

ALIFFIA MARLIANI FIRDAUS, - (2024) DERIVATISASI KUERSETIN DENGAN BENZIL KLORIDA MELALUI REAKSI ETERIFIKASI. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Kuersetin merupakan senyawa flavonoid yang memiliki berbagai aktivitas farmakologi diantaranya antimikroba, antioksidan, dan antiinflamasi. Penelitian ini dilakukan untuk menghasilkan turunan kuersetin dengan benzil klorida melalui reaksi eterifikasi. Derivatisasi dilakukan menggunakan dua pelarut berbeda yaitu DMF dan metanol dengan reagen benzil klorida dan katalis piperidin. Hasil reaksi dipisahkan dengan Ekstraksi Cair-Cair (ECC) dan dimurnikan dengan kromatografi kolom. Identifikasi derivat menggunakan KLT, spektrofotometri UV-Visibel, dan spektrofotometri IR. Hasil rendemen pada derivat dengan pelarut DMF adalah 41,62% sedangkan metanol 26,32%. Identifikasi derivat dengan KLT menunjukkan perubahan nilai Rf. Spektrum UV-Visibel menunjukkan pergeseran panjang gelombang menjadi 257 nm (puncak pertama) dan 350 nm (puncak kedua) untuk derivat A sedangkan untuk derivat B menjadi 258 nm dan 289 nm dibandingkan dengan kuersetin 256 nm dan 352 nm. Spektrofotometri IR menunjukkan perbedaan bilangan gelombang dan penambahan gugus baru. Namun, pada derivat terjadi penurunan aktivitas antioksidan dengan nilai IC50 14 µg/mL (derivat A) dan 148 µg/mL (derivat B) dibandingkan dengan kuersetin yang memiliki nilai IC50 6 µg/mL. Derivatisasi dengan pelarut metanol menunjukkan perubahan yang lebih signifikan dibandingkan dengan DMF. ------ Quercetin is a flavonoid compound that has various pharmacological activities including antimicrobial, antioxidant, and anti-inflammatory. This study was conducted to produce quercetin derivatives with benzyl chloride through etherification reactions. Derivatization was carried out using two different solvents, namely DMF and methanol with benzyl chloride reagent and piperidine catalyst. The reaction results were separated by Liquid-Liquid Extraction (ECC) and purified by column chromatography. Derivative identification using TLC, UVVisible spectrophotometry, and IR spectrophotometry. The yield of the derivative with DMF solvent was 41.62% while methanol was 26.32%. Derivative identification with TLC showed changes in Rf values. The UV-Visible spectrum showed a wavelength shift to 257 nm (first peak) and 350 nm (second peak) for derivative A while for derivative B it became 258 nm and 289 nm compared to quercetin 256 nm and 352 nm. IR spectrophotometry showed differences in wave numbers and the addition of new groups. However, in the derivatives there was a decrease in antioxidant activity with IC50 values of 14 µg/mL (derivative A) and 148 µg/mL (derivative B) compared to quercetin with an IC50 value of 6 µg/mL. Derivatization with methanol solvent showed more significant changes compared to DMF.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	kuersetin, derivatisasi, eterifikasi, benzil klorida. ----- Quercetin, derivatization, etherification, benzyl chloride
Subjects:	Q Science > QD Chemistry R Medicine > R Medicine (General)
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	28 Aug 2024 04:23
Last Modified:	28 Aug 2024 04:23
URI:	http://repository.stfi.ac.id/id/eprint/1094

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