NURUL PADILAH, - (2024) PENETAPAN KADAR FLAVONOID TOTAL DARI EKSTRAK AIR DAUN SUKUN (Artocarpus altilis (Parkinson) fosberg), BUNGA ROSELLA (Hibiscus sabdariffa L.) DAN KOMBINASI. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Tumbuhan obat merupakan keanekaragaman hayati yang menjadi salah satu alternatif pengobatan yang terjangkau untuk Masyarakat Indonesia, flavonoid adalah suatu senyawa metabolit sekunder yang terbesar dalam dunia tumbuhan yang berpotensi sebagai antioksidan, tanaman yang diketahui memiliki kandungan senyawa flavonoid adalah daun sukun dan bunga rosella. Tujuan penelitian ini untuk mengetahui profil kromatografi lapis tipis (KLT), kadar flavonoid total, dan mengetahui berbedaan hasil yang signifikan dari daun sukun, bunga rosella dan perbandingan antara keduanya (1:1, 1:2, 2:1) dengan menggunakan spektrofotometer UV-Vis dan metode statistik ANOVA satu arah (one-way). Didapat hasil pada plat KLT berfluoresensi kuning kehijauan atau biru pada dibawah lampu UV 366 nm, pada uji kadar flavonoid total didapat regresi linier baku standar kuersetin y= 0,1273x + 0,0036; R² = 0,9968, hasil penelitian ini menunjukkan hasil ekstrak daun sukun dan bunga rosella memiliki kandungan flavonoid dengan kadar terbesar terdapat pada perbandingan 1:2 (sukun:rosella) dengan kadar sebesar 136,973 µg/gram ekstrak, dimana untuk kadar flavonoid dari daun sukun sendiri sebesar 95,863 µg/gram ekstrak, dan bunga rosella 128,332 µg/gram ekstrak, serta pada uji analisis statistik didapat perbedaan yang signifikan antara hasil kadar daun sukun, bunga rosella dan perbandingan antara keduanya (1:1, 1:2, 2:1). ----- Medicinal plants are a form of biodiversity and represent an accessible alternative treatment for the Indonesian community. Flavonoids are secondary metabolites that are abundant in the plant world and have potential as antioxidants. Plants known to contain flavonoid compounds include breadfruit leaves and hibiscus flowers. This study aims to determine the thin-layer chromatography (TLC) profile, total flavonoid content, and significant differences in the results from breadfruit leaves, hibiscus flowers, and their combinations (1:1, 1:2, 2:1) using UV-Vis spectroscopy and one-way ANOVA statistical methods. The results on the TLC plate showed yellow-green or blue fluorescence under UV light at 366 nm. The total flavonoid content test resulted in a quercetin standard calibration curve with a linear regression of y = 0.1273x + 0.0036; R² = 0.9968. This study found that the highest flavonoid content was in the 1:2 (breadfruit:hibiscus) extract with a concentration of 136.973 µg/gram of extract. The flavonoid content of breadfruit leaves alone was 95.863 µg/gram of extract, and hibiscus flowers had 128.332 µg/gram of extract. Statistical analysis revealed significant differences among the flavonoid levels in breadfruit leaves, hibiscus flowers, and their various combinations (1:1, 1:2, 2:1).
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Flavonoid, Daun sukun, Bunga rosella, Spektrofotometer UV-Vis, Kromatografi Lapis Tipis (KLT). ----- Flavonoids, Breadfruit leaves, Hibiscus flowers, UV-Vis Spectroscopy, Thin-Layer Chromatography (TLC) |
| Subjects: | Q Science > Q Science (General) R Medicine > RV Botanic, Thomsonian, and eclectic medicine |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 28 Aug 2024 03:28 |
| Last Modified: | 28 Aug 2024 03:28 |
| URI: | http://repository.stfi.ac.id/id/eprint/1088 |
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