KHANIA ZAVELLA, - (2024) UJI AKTIVITAS ANTIOKSIDAN DENGAN METODE DPPH (2,2-Diphenyl-1-Picrylhydrazil) PADA EKSTRAK DAUN SUKUN KUNING JATUH (Artocarpus altilis (Parkinson) Fosberg), BUNGA ROSELLA (Hibiscus sabdariffa L.) DAN KOMBINASI. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Senyawa antioksidan memiliki peran penting dalam kesehatan yang dapat melindungi tubuh terhadap kerusakan yang disebabkan oleh radikal bebas. Adapun tanaman yang berpotensi sebagai antioksidan alami adalah daun sukun (Artocarpus altilis (Parkinson) Fosberg) dan bunga rosella (Hibiscus sabdariffa L.). Penelitian ini bertujuan untuk menguji aktivitas antioksidan dari ekstrak kering daun sukun kuning jatuh (Artocarpus altilis (Parkinson) Fosberg), bunga rosella (Hibiscus sabdariffa L.) dan kombinasi menggunakan metode DPPH (2,2-Diphenyl-1- Picrylhydrazil). Penelitian ini diawali dengan Ekstraksi dengan pelarut aquadest menggunakan metode dekok dan diubah menjadi ekstrak kering menggunakan spraydryer, karakterisasi simplisia, skrining fitokimia, kromatografi lapis tipis (KLT) dan identifikasi aktivitas antioksidan menggunakan spektrofotometri UVVisible. Hasil uji fitokimia menunjukkan bahwa simplisia dan ekstrak daun sukun mengandung tanin, flavonoid, saponin, triterpenoid dan fenol sedangkan bunga rosella mengandung tanin, flavonoid, saponin dan fenol. Hasil pengujian secara kulitatif dengan Kromatografi Lapis Tipis (KLT) menunjukan adanya senyawa antioksidan pada ke lima sampel yang ditandai dengan noda berwarna kuning latar ungu setelah disemprot penampak bercak DPPH 0,2%. Hasil pengujian aktivitas antioksidan secara kuantitatif dengan metode DPPH menunjukkan bahwa aktivitas antioksidan nilai IC50 tertinggi 540,5 ppm pada daun sukun:bunga rosella (2:1) dan terendah 880,6 ppm pada bunga rosella. ------ Antioxidant compounds have an important role in health that can protect the body against damage caused by free radicals. Plants that have the potential as natural antioxidants are breadfruit leaves (Artocarpus altilis (Parkinson) Fosberg) and rosella flowers (Hibiscus sabdariffa L.). This study aims to test the antioxidant activity of dry extracts of yellow fallen breadfruit leaves (Artocarpus altilis (Parkinson) Fosberg), rosella flowers (Hibiscus sabdariffa L.) and combinations using the DPPH method (2,2-Diphenyl-1-Picrylhydrazil). This study began with extraction with aquadest solvent using the decok method and converted into dry extract using a spray dryer, characterization of simplicia, phytochemical screening, thin layer chromatography (TLC) and identification of antioxidant activity using UV-Visible spectrophotometry. The results of the phytochemical test showed that the simplicia and breadfruit leaf extract contained tannins, flavonoids, saponins, triterpenoids and phenols while rosella flowers contained tannins, flavonoids, saponins and phenols. The results of qualitative testing with Thin Layer Chromatography (TLC) showed the presence of antioxidant compounds in the five samples which were marked with yellow spots on a purple background after being sprayed with 0.2% DPPH spot detector. The results of quantitative antioxidant activity testing with the DPPH method showed that the highest IC50 value of antioxidant activity was 540.5 ppm in breadfruit leaves: rosella flowers (2:1) and the lowest was 880.6 ppm in rosella flowers.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Daun sukun, bunga rosella, antioksidan, DPPH, KLT. ----- Breadfruit leaves, rosella flowers, antioxidants, DPPH, TLC. |
| Subjects: | Q Science > Q Science (General) R Medicine > RV Botanic, Thomsonian, and eclectic medicine |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 28 Aug 2024 03:06 |
| Last Modified: | 28 Aug 2024 03:06 |
| URI: | http://repository.stfi.ac.id/id/eprint/1085 |
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