Isolation and identification of proteolytic bacteria from pig sludge and protease activity determination

SYARIF HAMDANI, - and NISA ASSTIYANI, - and DEWI ASTRIANY, - and MARLIA SINGGIH, - and SLAMET IBRAHIM, - (2019) Isolation and identification of proteolytic bacteria from pig sludge and protease activity determination. IOP Conference Series: Earth and Environmental Science, 230 (1). pp. 1-7. ISSN 1755-1315

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Abstract

Proteolytic bacteria were found in many environment, some were found in soil or mud. This research was conducted to find proteolytic bacteria from pig sludge that is place for pig wallowing. Bacteria isolation was carried out by streak plate method on nutrient agar media. Qualitative test of proteolytic bacteria used skim milk agar medium. The results of isolation obtained 10 bacterias and 2 of them have potential as proteolytic bacteria. Identification of proteolitic bacteria used the 16s rRNA method, result showed that bacterias are Bacillus pseudomycoides and Staphylococcus sciuri. Protease isolation was carried out under 4°C and under room temperature during centrifugation as separation process. Protease activity from Bacillus pseudomycoides was at 4°C as much as 0.094 U/mL and was 0.082 U/mL at room temperature, while from Staphylococcus sciuri was at 4°C as much as 0.120 U/mL and was 0.098 U/mL at room temperature.

Item Type:	Article
Uncontrolled Keywords:	Isolation, proteolytic bacteria, protease, activity
Subjects:	Q Science > QR Microbiology
Divisions:	Lembaga Penelitian dan Pengabdian Masyarakat > Publikasi Penelitian
Depositing User:	- Dewi Astriany -
Date Deposited:	20 Aug 2024 07:14
Last Modified:	20 Aug 2024 07:14
URI:	http://repository.stfi.ac.id/id/eprint/968

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