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	<title>Stabilitas Media dan Viabilitas Hansenula polymorpha untuk Produksi Vaksin Hepatitis B rekombinan | Astriany | Prosiding Seminar Nasional Diseminasi Penelitian Volume 3</title>
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	<meta name="DC.Contributor.Sponsor" xml:lang="en" content=""/>
	<meta name="DC.Creator.PersonalName" content="Dewi Astriany"/>
	<meta name="DC.Creator.PersonalName" content="Erman Tritama"/>
	<meta name="DC.Creator.PersonalName" content="Nurus Soliha"/>
	<meta name="DC.Creator.PersonalName" content="Dhianti Kiky Kandea Dewi"/>
	<meta name="DC.Date.created" scheme="ISO8601" content="2023-10-08"/>
	<meta name="DC.Date.dateSubmitted" scheme="ISO8601" content="2023-10-06"/>
	<meta name="DC.Date.issued" scheme="ISO8601" content="2023-10-05"/>
	<meta name="DC.Date.modified" scheme="ISO8601" content="2023-10-06"/>
	<meta name="DC.Description" xml:lang="en" content=" Pendahuluan : Salah satu langkah pencegahan terjadinya infeksi akibat virus Hepatitis B adalah dengan pemberian vaksin. Vaksin Hepatitis B rekombinan dapat diproduksi menggunakan sel inang ragi  Hansenula polymorpha . Produksi vaksin ini dipengaruhi oleh banyak faktor di antaranya kondisi media dan pertumbuhan sel inang.  Tujuan : Penelitian ini dilakukan untuk mengetahui umur media yang masih optimal untuk pertumbuhan  H. polymorpha  dengan mengamati nilai  optical density  dan viabilitasnya, serta untuk mengetahui pengaruh proses  freeze-thaw  terhadap stabilitas  H. polymorpha .  Metode : Preservasi  H. polymorpha  dilakukan dengan menyimpanan dalam larutan gliserol 15% pada suhu -80ºC.  H. polymorpha  diberi perlakuan siklus  freeze-thaw  sebanyak 1x, 2x, 3x, 4x, 5x. Nilai  optical density  dan nilai viabilitas  H. polymorpha  yang ditumbuhkan pada media SYN6 diamati pada hari ke-2, ke-4, ke-6, ke-8, dan ke-10.  Hasil : Pada hari ke-8 Hansenula polymorpha telah mencapai batas pertumbuhannya pada pH 4 dengan nilai viabilitas 34x10 7  cfu.  Kesimpulan : Dari data tersebut dapat disimpulkan bahwa media SYN6 dapat digunakan sebagai media pertumbuhan  H. polymorpha  yang baik hingga hari ke-6 penyimpanan dan  H. polymorpha  tetap stabil setelah 5 kali siklus  freeze-thaw ."/>
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	<meta name="DC.Source.Volume" content="3"/>	<meta name="DC.Title" content="Stabilitas Media dan Viabilitas Hansenula polymorpha untuk Produksi Vaksin Hepatitis B rekombinan"/>
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	<meta name="citation_journal_title" content="Prosiding Seminar Nasional Diseminasi Penelitian Volume 3"/>
        <meta name="citation_author" content="Dewi Astriany"/>
        <meta name="citation_author" content="Erman Tritama"/>
        <meta name="citation_author" content="Nurus Soliha"/>
        <meta name="citation_author" content="Dhianti Kiky Kandea Dewi"/>
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