MEGA PORIANINGSIH PANGGABEAN, - (2021) STUDI IN SILICO PENENTUAN EPITOP DAN PEMODELAN STRUKTUR ANTIGEN FILAMENTOUS HEMAGLUTININ, SEROTIPE FIMBRIAE 2 DAN 3, SERTA PERTACTIN DARI Bordetella pertussis. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Pertussis atau whooping cough merupakan penyakit yang disebabkan oleh bakteri Bordetella pertussis. Di Indonesia vaksin pertussis masih diberikan dalam bentuk whole cell, dimana vaksin tersebut memiliki efek samping seperti menangis terus menerus selama 3 jam atau lebih, kejang, kejadian hipotonik-hiporesponsif dan ensefalopati. Tujuan dari penelitian ini untuk menentukan epitop dan memodelkan struktur dari antigen Filamentous Hermaglutinin, Pertactin, serta Serotipe Fimbriae 2 dan 3 dalam pengembangan vaksin aseluler pertussis berbasis bioinformatika. Metode yang dilakukan diawali dengan mencari urutan asam amino antigen Bordetella pertussis kemudian masing-masing antigen dilakukan pencarian epitop linear, aksesibilitas, fleksibilitas serta dilakukan pemodelan struktur setiap antigen. Secara keseluruhan setiap antigen memiliki kandidat epitop linear, aksesibilitas serta fleksibilitas yang telah yang memenuhi standar parameter software yaitu nilai sama dengan 1 atau lebih dari 1. Dari pemodelan struktur dihasilkan 3 epitop discontinues pada antigen Filamentous Hemaglutinin, 3 epitop discontinues pada antigen Pertactin, 2 epitop discontinues pada antigen Serotipe Fimbriae 2 dan 6 epitop discontinues pada antigen Serotipe Fimbriae 3 yang akan berikatan dengan antibodi. ----- Pertussis or whooping cough is a disease caused by the bacterium Bordetella pertussis. In Indonesia the pertussis vaccine is still given in whole cell form, where the vaccine has side effects such as crying continuously for 3 hours or more, seizures, hypotonic-hyporesponsive events and encephalopathy. The purpose of this study was to determine the epitope and model the structure of the Filamentous Hemagglutinin, Pertactin, and Fimbriae Serotypes 2 and 3 antigens in the development of bioinformatics-based acellular pertussis vaccines. The method started by searching for the amino acid sequence of the Bordetella pertussis antigen, then each antigen was searched for linear epitope, accessibility, flexibility and modeling of each antigen was carried out. Overall, each antigen has a linear epitope candidate, accessibility and flexibility that meet the standard software parameters, namely a value equal to 1 or more than 1. From the structural modeling, 3 epitopes are produced on the Filamentous Hemagglutinin antigen, 3 epitopes stop on the Pertactin antigen, 2 epitopes stop on the Fimbriae Serotype 2 antigen and 6 epitopes stop on the Fimbriae 3 Serotype antigen which will bind to the antibody.
| Item Type: | Thesis (Skripsi) |
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| Uncontrolled Keywords: | Aseluler vaksin, Bioinformatika, Epitop, Aksesibilitas dan Fleksibilitas, Pemodelan Struktur. ----- Acellular vaccine, Bioinformatics, Epitope, Accessibility and Flexibility, Structural Modeling |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 16 Aug 2024 06:30 |
| Last Modified: | 16 Aug 2024 06:30 |
| URI: | http://repository.stfi.ac.id/id/eprint/842 |
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