AURA ANDINA RAHAYU, - (2026) PENGARUH JENIS BIO-ELISITOR BERBASIS FUNGI ENDOFIT (Clasdoparium sp) TERHADAP KADAR ANDROGRAFOLID PADA KULTUR SUSPENSI SEL SAMBILOTO (Andrographis Paniculata). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Sambiloto (Andrographis paniculata) merupakan tanaman obat penghasil andrografolid, senyawa diterpen lakton dengan berbagai aktivitas farmakologi, namun produksinya secara konvensional masih rendah dan membutuhkan waktu lama. Kultur suspensi sel dengan elisitasi bio-elisitor fungi endofit merupakan pendekatan alternatif untuk mempercepat produksi andrografolid. Penelitian ini bertujuan menganalisis pengaruh bio-elisitor Cladosporium sp berupa larutan fermentasi (LF) dan larutan miselium (LM) terhadap pertumbuhan kalus dan kadar andrografolid pada kultur suspensi sel A. paniculata, dibandingkan kontrol negatif dan kontrol positif asam jasmonat (AJ). Kultur suspensi sel dielisitasi selama 24 jam pada fase stasioner, dianalisis menggunakan KCKT, dan data dianalisis dengan uji Welch ANOVA. Hasil menunjukkan LF menghasilkan bobot basah (0,338 g) dan bobot kering (0,037 g) lebih tinggi serta kadar air (89,05%) lebih rendah dibandingkan LM (0,301 g; 0,030 g; 90,03%), sehingga LF efektif meningkatkan biomassa kalus dibandingkan LM. Namun, kadar andrografolid tidak berbeda signifikan antara LF, LM, maupun kontrol (p = 0,498 > 0,05) dan masih berada di bawah batas kuantifikasi. Dengan demikian, hasil menunjukkan bahwa bio-elisitor Cladosporium sp mampu meningkatkan pertumbuhan biomassa kalus, tetapi elisitasi selama 24 jam belum optimal untuk meningkatkan akumulasi andrografolid pada kultur suspensi sel sambiloto. ----- Andrographis paniculata (sambiloto) is a medicinal plant producing andrographolide, a diterpene lactone with various pharmacological activities, although its conventional production remained low and required a long cultivation period. Cell suspension culture elicited with fungal endophyte-based bio-elicitors was applied as an alternative approach to accelerate andrographolide production. This study aimed to analyze the effect of Cladosporium sp bio-elicitors, namely fermentation solution (FS) and mycelium solution (MS), on callus growth and andrographolide content in A. paniculata cell suspension culture, compared with negative and positive (jasmonic acid) controls. The culture was elicited for 24 hours at the stationary phase, analyzed using HPLC, and the data were statistically analyzed using Welch ANOVA. The results showed that FS produced higher fresh weight (0.338 g) and dry weight (0.037 g) with lower water content (89.05%) than MS (0.301 g; 0.030 g; 90.03%), indicating that FS was more effective than MS in promoting callus biomass growth. However, andrographolide content did not differ significantly among FS, MS, and the controls (p = 0.498 > 0.05) and remained below the quantification limit. It was concluded that fermentation solution was to mycelium solution in promoting callus biomass growth, but both bio-elicitors were not yet effective in increasing andrographolide content after 24 hours of elicitation.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | sambiloto, andrografolid, bio-elisitor, Cladosporium sp, kultur suspensi sel. ----- Andrographis paniculata, andrographolide, bio-elicitor, Cladosporium sp, cell suspension culture |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 23 Sep 2026 07:32 |
| Last Modified: | 23 Sep 2026 07:32 |
| URI: | http://repository.stfi.ac.id/id/eprint/4288 |
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