CHANDRA YUDHA DWI CAHYANTO, - (2026) EVALUASI METODE ANALISIS 2,5-HEKSANADION DALAM URIN DENGAN PENDEKATAN STANDAR INTERNAL MENGGUNAKAN GAS CHROMATOGRAPHY-FLAME IONIZATION DETECTOR (GC-FID). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
![[img]](http://repository.stfi.ac.id/style/images/fileicons/text.png) |
Text
S_PSSF_A221049_Title.pdf Download (551kB) |
|
Text
S_PSSF_A221049_Chapter1.pdf Restricted to Repository staff only Download (249kB) |
|
Text
S_PSSF_A221049_Chapter2.pdf Restricted to Repository staff only Download (563kB) |
|
Text
S_PSSF_A221049_Chapter3.pdf Restricted to Repository staff only Download (249kB) |
|
Text
S_PSSF_A221049_Chapter4.pdf Restricted to Repository staff only Download (423kB) |
|
Text
S_PSSF_A221049_Chapter5.pdf Restricted to Repository staff only Download (226kB) |
|
Text
S_PSSF_A221049_Appendix.pdf Restricted to Repository staff only Download (1MB) |
Abstract
N-heksana merupakan salah satu pelarut organik yang sering dimanfaatkan di berbagai sektor industri, namun penggunaannya sering kali berkaitan dengan risiko kesehatan yang serius. Paparan n-heksana di lingkungan kerja berisiko menyebabkan neuropati perifer akibat metabolit utamanya, 2,5-heksanadion yang diekskresikan melalui urin. Penelitian ini bertujuan mengembangkan dan memvalidasi metode analisis 2,5-heksanadion dalam urin menggunakan Gas Chromatography-Flame Ionization Detector (GC-FID) dengan pendekatan standar internal untuk biomonitoring okupasional. Kuantifikasi dilakukan menggunakan Gas Chromatography-Flame Ionization Detector (GC-FID) dengan penambahan standar internal yang difokuskan pada optimasi sistem kromatografi dan evaluasi linearitas, akurasi, presisi, Limit of Detection (LoD), Limit of Quantitation (LoQ), serta ketahanan (robustness). Hasil pengujian menunjukkan uji kesesuaian sistem memenuhi kriteria dengan %CV waktu retensi 0,00602%, jumlah lempeng teoritis (N) 329.716,881, dan resolusi (Rs) 4,275. Metode ini menghasilkan linearitas yang sangat baik pada rentang 0,05–1,6 ppm (r = 0,9996), akurasi (% recovery) 90,595%- 99,101%, serta presisi (%RSD) 3,32%-7,15%. Nilai LoD sebesar 0,0575 mg/L dan LoQ sebesar 0,1742 mg/L berada di bawah batas biologis ACGIH, dengan suhu awal oven 30°C sebagai kondisi paling optimal pada uji ketahanan. Disimpulkan bahwa metode GC-FID berbasis standar internal sikloheksanon terbukti valid, akurat, presisi, dan sensitif untuk kuantifikasi 2,5-heksanadion dalam urin. ------ N-hexane is an organic solvent widely used across industrial sectors, but its use carries serious health risks. Occupational exposure to n-hexane can cause peripheral neuropathy due to its major metabolite, 2,5-hexanedione, excreted in urine. This study aimed to develop and validate a method for determining 2,5- hexanedione in urine using Gas Chromatography-Flame Ionization Detector (GCFID) with an internal standard approach for occupational biomonitoring. Quantification used GC-FID with an added internal standard, focusing on chromatographic system optimization and evaluation of linearity, accuracy, precision, Limit of Detection (LoD), Limit of Quantitation (LoQ), and robustness. Results showed the system suitability test met acceptance criteria, with a retention time %CV of 0.00602%, theoretical plate number (N) of 329.716,881, and resolution (Rs) of 4,275. The method showed excellent linearity over 0,05–1,6 ppm (r = 0,9996), accuracy (% recovery) of 90,595%-99,101%, and precision (%RSD) of 3,32%-7,15%. LoD and LoQ values were 0,0575 mg/L and 0,1742 mg/L, both below the ACGIH biological exposure index, with an initial oven temperature of 30°C as the optimal robustness condition. The cyclohexanone-based internal standard GC-FID method proved valid, accurate, precise, and sensitive for quantifying 2,5-hexanedione in urine.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | 2,5-heksanadion, GC-FID, Sikloheksanon, Urin, Validasi. ------- 2,5-hexanedione, GC-FID, Cyclohexanone, Urine, Validation |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 23 Sep 2026 06:45 |
| Last Modified: | 23 Sep 2026 06:45 |
| URI: | http://repository.stfi.ac.id/id/eprint/4279 |
Actions (login required)
 |
View Item |
