STUDI IN SILICO PEPTIDA ANTIMIKROBA DARI KAYU SECANG (CAESALPINIA SAPPAN L.) TERHADAP KLEBSIELLA PNEUMONIAE

HANIF FARID SUDJUD, - (2026) STUDI IN SILICO PEPTIDA ANTIMIKROBA DARI KAYU SECANG (CAESALPINIA SAPPAN L.) TERHADAP KLEBSIELLA PNEUMONIAE. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Klebsiella pneumoniae merupakan bakteri Gram-negatif anggota ESKAPE yang resistensinya terhadap antibiotik terus meningkat. Kondisi ini menuntut alternatif terapi baru seperti peptida antimikroba. Penelitian ini bertujuan mengidentifikasi dan mengevaluasi kandidat peptida antimikroba dari kayu secang terhadap tiga protein target K. pneumoniae yaitu OmpA, SHV-1 β-laktamase, dan OmpK36. Penelitian dilakukan melalui pendekatan in silico. Tahapan penelitian meliputi identifikasi peptida dari sekuens protein kayu secang serta prediksi aktivitas antimikroba, sifat fisikokimia, sifat biologis, dan profil ADMET. Struktur tiga dimensi peptida diprediksi dan divalidasi sebelum ditambatkan pada protein target dengan meropenem dan VvK1 sebagai kontrol. Dari tujuh peptida yang diuji, peptida LGITK dan LGITKT terpilih sebagai dua kandidat terbaik. Keduanya memiliki aktivitas antimikroba spesifik, sifat fisikokimia stabil, profil biologis dan ADMET yang aman, serta struktur tiga dimensi tervalidasi baik. Kedua peptida menunjukkan interaksi molekuler dan afinitas ikatan yang baik terhadap seluruh protein target. Peptida LGITKT secara konsisten lebih unggul dan SHV-1 βlaktamase merupakan target paling prospektif karena afinitas ikatan kedua peptida melampaui kontrol meropenem dan VvK1. Temuan ini menunjukkan bahwa peptida LGITK dan LGITKT merupakan kandidat peptida antimikroba potensial terhadap K. pneumoniae. ------- Klebsiella pneumoniae is a Gram-negative bacterium of the ESKAPE group whose resistance to antibiotics continues to increase, demanding new therapeutic alternatives such as antimicrobial peptides. This study aimed to identify and evaluate candidate antimicrobial peptides derived from sappan wood (Caesalpinia sappan L.) against three K. pneumoniae target proteins, namely OmpA, SHV-1 βlactamase, and OmpK36. The research was conducted using an in silico approach. The stages included identification of peptides from sappan wood protein sequences, followed by prediction of antimicrobial activity, physicochemical properties, biological properties, and ADMET profile. Three-dimensional peptide structures were predicted and validated prior to docking onto the target proteins, with meropenem and VvK1 used as controls. Of the seven peptides tested, LGITK and LGITKT were selected as the two best candidates. Both exhibited specific antimicrobial activity, stable physicochemical properties, safe biological and ADMET profiles, and well-validated three-dimensional structures. Both peptides demonstrated favorable molecular interactions and binding affinities toward all target proteins. LGITKT consistently outperformed LGITK, and SHV-1 βlactamase was identified as the most prospective target, as the binding affinities of both peptides exceeded those of the meropenem and VvK1 controls. These findings indicated that LGITK and LGITKT were potential antimicrobial peptide candidates against K. pneumoniae.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	antimikroba, Caesalpinia sappan L., Klebsiella pneumoniae, peptida. ----- antimicrobial, Caesalpinia sappan L., Klebsiella pneumoniae, peptide
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	23 Sep 2026 04:37
Last Modified:	23 Sep 2026 04:37
URI:	http://repository.stfi.ac.id/id/eprint/4276

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