AGUSTINA ASTRINITAS MELANI WAGO, - (2026) UJI SITOTOKSISITAS EKSTRAK ETANOL DAUN PEPAYA JEPANG (Cnidoscolus aconitifolius (Mill.) I.M. Johnst.) TERHADAP LINI SEL KANKER PARU-PARU A549 DENGAN METODE RESAZURIN. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Kanker paru-paru merupakan salah satu penyebab utama kematian akibat kanker di dunia, sehingga diperlukan pengembangan kandidat antikanker baru yang berasal dari bahan alam. Daun pepaya jepang (Cnidoscolus aconitifolius (Mill.) I.M. Johnst.) diketahui mengandung berbagai senyawa metabolit sekunder, seperti flavonoid, alkaloid, dan fenolik yang berpotensi memiliki aktivitas antikanker. Penelitian ini bertujuan untuk mengetahui aktivitas sitotoksik dan menentukan nilai IC50 ekstrak etanol daun pepaya jepang terhadap sel kanker paru-paru A549 menggunakan metode Resazurin. Ekstrak diperoleh melalui metode maserasi menggunakan etanol 70%, kemudian dilakukan karakterisasi simplisia, skrining fitokimia, dan pengujian sitotoksisitas terhadap sel kanker paru-paru A549 dengan variasi konsentrasi ekstrak 7,81–1000 µg/mL. Pengukuran viabilitas sel dilakukan menggunakan reagen PrestoBlue™, sedangkan nilai IC50 ditentukan berdasarkan kurva dose-response menggunakan perangkat lunak GraphPad Prism. Hasil skrining fitokimia menunjukkan bahwa ekstrak etanol daun pepaya jepang mengandung alkaloid, fenolik, tanin, flavonoid, saponin, steroid, dan triterpenoid. Hasil uji sitotoksisitas menunjukkan bahwa ekstrak etanol daun pepaya jepang memberikan efek sitotoksik terhadap sel A549 secara dose-dependent. Nilai IC₅₀ yang diperoleh sebesar 986,70 µg/mL dan berdasarkan klasifikasi aktivitas sitotoksik termasuk dalam kategori tidak sitotoksik terhadap sel kanker paru-paru A549. Dengan demikian, ekstrak etanol daun pepaya jepang belum menunjukkan aktivitas sitotoksik yang kuat terhadap sel kanker paru-paru A549. ------ Lung cancer is one of the leading causes of cancer-related mortality worldwide, highlighting the need for the development of new anticancer candidates derived from natural products. Japanese papaya leaves (Cnidoscolus aconitifolius (Mill.) I.M. Johnst.) are known to contain various secondary metabolites, such as flavonoids, alkaloids, and phenolic compounds, which have potential anticancer activity. This study aimed to evaluate the cytotoxic activity and determine the IC50 value of ethanol extract of japanese papaya leaves against A549 lung cancer cells using the Resazurin method. The extract was obtained through maceration using 70% ethanol, followed by crude drug characterization, phytochemical screening, and cytotoxicity testing against A549 lung cancer cells at extract concentrations ranging from 7.81–1000 µg/mL. Cell viability was measured using PrestoBlue™ reagent, while the IC₅₀ value was determined based on the dose-response curve using GraphPad Prism software. Phytochemical screening revealed that the ethanol extract of japanese papaya leaves contained alkaloids, phenolics, tannins, flavonoids, saponins, steroids, and triterpenoids. The cytotoxicity assay showed that the ethanol extract of japanese papaya leaves exhibited a dose-dependent cytotoxic effect against A549 cells. The obtained IC50 value was 986.70 µg/mL, which was classified as non-cytotoxic activity against A549 lung cancer cells based on cytotoxicity activity classification. Therefore, the ethanol extract of japanese papaya leaves did not exhibit strong cytotoxic activity against A549 lung cancer cells.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | kanker, paru-paru, pepaya jepang, sitotoksisitas. ------ cytotoxicity, cancer, lung, japanese papaya |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 23 Sep 2026 02:53 |
| Last Modified: | 23 Sep 2026 02:53 |
| URI: | http://repository.stfi.ac.id/id/eprint/4270 |
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