NUR ASTRI SUSILAWATI, - (2026) PENGARUH 2,4-DICHLOROPHENOXYACETIC ACID (2,4-D) DAN 6-BENZYL AMINO PURINE (BAP) SERTA ELISITOR ASAM JASMONAT TERHADAP KADAR ANDROGRAFOLID SAMBILOTO SECARA IN VITRO. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
![[img]](http://repository.stfi.ac.id/style/images/fileicons/text.png) |
Text
S_PSSF_A221060_Title.pdf Download (692kB) |
|
Text
S_PSSF_A221060_Chapter1.pdf Restricted to Repository staff only Download (206kB) |
|
Text
S_PSSF_A221060_Chapter2.pdf Restricted to Repository staff only Download (398kB) |
|
Text
S_PSSF_A221060_Chapter3.pdf Restricted to Repository staff only Download (193kB) |
|
Text
S_PSSF_A221060_Chapter4.pdf Restricted to Repository staff only Download (419kB) |
|
Text
S_PSSF_A221060_Chapter5.pdf Restricted to Repository staff only Download (178kB) |
|
Text
S_PSSF_A221060_Appendix.pdf Restricted to Repository staff only Download (1MB) |
Abstract
Sambiloto (Andrographis paniculata) kaya akan senyawa andrografolid sebagai bahan baku obat, namun produksi secara konvensional terkendala oleh variasi iklim dan masa panen. Kultur jaringan melalui perbanyakan kalus remah dengan zat pengatur tumbuh serta proses elisitasi merupakan alternatif efisien untuk mengoptimalkan produksi metabolit sekunder secara terkontrol. Penelitian ini menganalisis induksi kalus remah eksplan daun sambiloto pada media Murashige and Skoog (MS) dengan variasi zat pengatur tumbuh (1 mg/L 2,4-D + 0,5 mg/L BAP), (1 mg/L 2,4-D + 1 mg/L BAP) serta (2 mg/L 2,4-D + 0,5 mg/L BAP) dilanjutkan elisitasi asam jasmonat 10 mg/L selama 7 hari, dan penetapan kadar andrografolid menggunakan Kromatografi Cair Kinerja Tinggi (KCKT). Induksi kalus remah terbaik dan tercepat yaitu pada hari ke-12 diperoleh pada perlakuan (2,4-D 1 mg/L + BAP 0,5 mg/L) dan (2,4-D 1 mg/L + BAP 1 mg/L) dengan warna hijau kekuningan. Pascaelisitasi, kadar andrografolid pada kombinasi 2,4-D 1 mg/L + BAP 1 mg/L meningkat dari 0,112% menjadi 0,115% (naik 3%). Sebaliknya, pada kombinasi 2,4-D 1 mg/L + BAP 0,5 mg/L terjadi penurunan dari 0,164% menjadi 0,123% (turun 25%), serta pada 2,4-D 2 mg/L + BAP 0,5 mg/L turun dari 0,111% menjadi 0,081% (turun 27%). Kondisi hormonal awal pada kalus berpengaruh secara signifikan terhadap efektivitas elisitasi asam jasmonat dalam merangsang akumulasi andrografolid. ------ Andrographis paniculata is rich in andrographolide compounds as pharmaceutical raw materials, yet its conventional production is constrained by climate variations and harvesting periods. Tissue culture through friable callus proliferation utilizing plant growth regulators combined with an elicitation process serves as an efficient alternative to optimize secondary metabolite production in a controlled manner. This study analyzed friable callus induction from Andrographis paniculata leaf explicants on Murashige and Skoog (MS) medium with variations of plant growth regulators (1 mg/L 2,4-D + 0.5 mg/L BAP), (1 mg/L 2,4-D + 1 mg/L BAP), and (2 mg/L 2,4-D + 0.5 mg/L BAP), followed by jasmonic acid elicitation at 10 mg/L for 7 days, and the determination of andrographolide content using High-Performance Liquid Chromatography (HPLC). The best and fastest friable callus induction, occurring on day 12, was achieved in treatments of (2,4-D 1 mg/L + BAP 0.5 mg/L) and (2,4-D 1 mg/L + BAP 1 mg/L) with yellowish-green characteristics. Postelicitation, the andrographolide content in the combination of 2,4-D 1 mg/L + BAP 1 mg/L increased from 0.112% to 0.115% (a 3% increase). Conversely, in the combination of 2,4-D 1 mg/L + BAP 0.5 mg/L, a decrease occurred from 0.164% to 0.123% (a 25% decrease), while in 2,4-D 2 mg/L + BAP 0.5 mg/L, it decreased from 0.111% to 0.081% (a 27% decrease). The initial hormonal state of the callus significantly affects the efficacy of jasmonic acid elicitation in stimulating andrographolide accumulation.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Andrographis paniculata, 2,4-D, BAP, Asam Jasmonat, Andrografolid, KCKT. ------- Andrographis paniculata, 2,4-D, BAP, Jasmonic Acid, Andrographolide, HPLC. |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 22 Sep 2026 03:07 |
| Last Modified: | 22 Sep 2026 03:07 |
| URI: | http://repository.stfi.ac.id/id/eprint/4260 |
Actions (login required)
 |
View Item |
