ASHRILYA DIANA PUTRI, - (2026) AKTIVITAS ANTIOKSIDAN DAN KORELASI KUALITATIF DENGAN KANDUNGAN SENYAWA BIOAKTIF FRAKSI EKSTRAK ETANOL BIJI SAWO WALANDA (POUTERIA CAMPECHIANA (KUNTH.)BAEHNI.). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Biji sawo walanda (Pouteria campechiana (Kunth.) Baehni.) mengandung metabolit sekunder yang berpotensi sebagai antioksidan alami. Namun, informasi mengenai aktivitas antioksidan setiap fraksi ekstrak dan hubungannya dengan golongan metabolit sekunder masih terbatas. Penelitian ini bertujuan menentukan aktivitas antioksidan fraksi ekstrak etanol biji sawo walanda serta mengkorelasikannya secara kualitatif dengan golongan metabolit sekunder. Fraksinasi dilakukan menggunakan metode ekstraksi cair-cair dengan pelarut nheksan, etil asetat, dan air. Masing-masing fraksi dianalisis melalui skrining fitokimia dan KLT-bioautografi, sedangkan aktivitas antioksidan ditentukan menggunakan metode DPPH pada panjang gelombang 517 nm menggunakan microplate reader. Hasil skrining fitokimia menunjukkan fraksi air dan etil asetat mengandung fenol, flavonoid, serta tanin, sedangkan fraksi n-heksan hanya mengandung steroid/triterpenoid. Fraksi air memiliki aktivitas antioksidan tertinggi dengan nilai IC₅₀ 0,36 ppm, diikuti fraksi etil asetat 0,53 ppm dan fraksi n-heksan 46,02 ppm. Analisis KLT-bioautografi menunjukkan korelasi kualitatif antara noda yang memiliki aktivitas antioksidan dengan golongan metabolit sekunder, yaitu fraksi n-heksan dan etil asetat ditunjukkan dengan Rf 0,38-0,5 (fenolik); 0,4-0,42 (flavonoid); 0,44 (steroid/triterpenoid) yang hanya terdapat pada fraksi n-heksan, sedangkan fraksi air ditunjukkan dengan Rf 0,62 (flavonoid dan fenol), 0,78 (fenol). Senyawa fenolik, flavonoid, steroid/triterpenoid diduga berperan penting terhadap aktivitas antioksidan fraksi ekstrak etanol biji sawo walanda. ------ Sapodilla seeds (Pouteria campechiana (Kunth.) Baehni.) contain secondary metabolites that have the potential as natural antioxidants. However, information regarding the antioxidant activity of each extract fraction and its relationship with secondary metabolite groups is still limited. This study aims to determine the antioxidant activity of the ethanol extract fraction of sapodilla seeds and correlate it qualitatively with secondary metabolite groups. Fractionation was carried out using the liquid-liquid extraction method with n-hexane, ethyl acetate, and water as solvents. Each fraction was analyzed through phytochemical screening and TLCbioautography, while antioxidant activity was determined using the DPPH method at a wavelength of 517 nm using a microplate reader. The results of phytochemical screening showed that the water and ethyl acetate fractions contained phenols, flavonoids, and tannins, while the n-hexane fraction only contained steroids/triterpenoids. The water fraction had the highest antioxidant activity with an IC₅₀ value of 0.36 ppm, followed by the ethyl acetate fraction of 0.53 ppm and the n-hexane fraction of 46.02 ppm. TLC-bioautography analysis showed a qualitative correlation between spots that had antioxidant activity with secondary metabolite groups, namely the n-hexane and ethyl acetate fractions indicated by Rf 0.38-0.5 (phenolic); 0.4-0.42 (flavonoid); 0.44 (steroid/triterpenoid) which were only found in the n-hexane fraction, while the water fraction was indicated by Rf 0.62 (flavonoid and phenol), 0.78 (phenol). Phenolic, flavonoid, steroid/triterpenoid compounds are thought to play an important role in the antioxidant activity of the ethanol extract fraction of sapodilla seeds.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Pouteria campechiana, antioksidan, flavonoid, fenolik. ----- Pouteria campechiana, antioxidant, flavonoids, phenolics. |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 19 Sep 2026 02:35 |
| Last Modified: | 19 Sep 2026 02:35 |
| URI: | http://repository.stfi.ac.id/id/eprint/4249 |
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