LUSIA NATALIA KEO, - (2026) EKSTRAKSI DAN FRAKSINASI SENYAWA ALKALOID PADA KULIT BATANG DADAP SEREP (Erytrina Subumbrans (Hassk.) Merr) DENGAN METODE ULTRASONIC ASSISTED EXTRACTION (UAE). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
![[img]](http://repository.stfi.ac.id/style/images/fileicons/text.png) |
Text
S_PSSF_A252087_Title.pdf Download (557kB) |
|
Text
S_PSSF_A252087_Chapter1.pdf Restricted to Repository staff only Download (29kB) |
|
Text
S_PSSF_A252087_Chapter2.pdf Restricted to Repository staff only Download (297kB) |
|
Text
S_PSSF_A252087_Chapter3.pdf Restricted to Repository staff only Download (835kB) |
|
Text
S_PSSF_A252087_Chapter4.pdf Restricted to Repository staff only Download (295kB) |
|
Text
S_PSSF_A252087_Chapter5.pdf Restricted to Repository staff only Download (258kB) |
|
Text
S_PSSF_A252087_Appendix.pdf Restricted to Repository staff only Download (894kB) |
Abstract
Senyawa alkaloid dari kulit batang Erythrina subumbrans (Hassk.) Merr. menggunakan metode Ultrasonic Assisted Extraction (UAE). Karakterisasi simplisia dilakukan melalui penetapan kadar sari larut air, kadar sari larut etanol, dan susut pengeringan. Identifikasi awal alkaloid dilakukan menggunakan pereaksi Mayer, Dragendorff, dan Wagner. Ekstraksi dilakukan menggunakan pelarut metanol, kemudian dilanjutkan dengan fraksinasi alkaloid menggunakan metode ekstraksi asam–basa untuk menghasilkan empat fraksi alkaloid. Selanjutnya, fraksi dianalisis menggunakan Kromatografi Lapis Tipis (KLT) dan spektrofotometri UV–Vis. Hasil karakterisasi simplisia menunjukkan kadar sari larut air sebesar 8,27%, kadar sari larut etanol 10,34%, dan susut pengeringan 4,81%, sehingga memenuhi persyaratan mutu simplisia. Ekstraksi menggunakan 1 kg simplisia dan 5 L metanol menghasilkan ekstrak kental sebanyak 99,36 g dengan rendemen sebesar 9,94%. Fraksinasi menghasilkan empat fraksi alkaloid dengan rendemen tertinggi pada Fraksi 1 sebesar 8,56%. Uji fitokimia menunjukkan hasil positif terhadap ketiga pereaksi alkaloid. Analisis KLT menunjukkan nilai Rf yang berbeda pada setiap fraksi, menandakan adanya variasi komponen alkaloid. Analisis spektrofotometri UV–Vis menunjukkan panjang gelombang maksimum pada kisaran 284–288 nm yang merupakan daerah serapan khas alkaloid golongan eritrinan. Hasil penelitian menunjukkan bahwa Erythrina subumbrans merupakan sumber alkaloid yang potensial dan metode UAE efektif digunakan untuk memperoleh fraksi alkaloid sebagai dasar penelitian fitokimia dan farmakologi. ------ This study aimed to extract, fractionate, and identify alkaloid compounds from the stem bark of Erythrina subumbrans (Hassk.) Merr. using the Ultrasonic Assisted Extraction (UAE) method. The crude drug was characterized by determining the water-soluble extractive value, ethanol-soluble extractive value, and loss on drying. Preliminary alkaloid screening was performed using Mayer, Dragendorff, and Wagner reagents. Extraction was conducted with methanol, followed by acid–base fractionation to obtain four alkaloid fractions. The fractions were further analyzed using Thin Layer Chromatography (TLC) and UV–Visible spectrophotometry. The characterization results showed water-soluble extractive, ethanol-soluble extractive, and loss on drying values of 8.27%, 10.34%, and 4.81%, respectively, indicating that the crude drug met the required quality standards. Extraction of 1 kg of powdered stem bark with 5 L of methanol produced 99.36 g of crude extract with a yield of 9.94%. Fractionation generated four alkaloid fractions, with Fraction 1 showing the highest yield (8.56%). TLC analysis revealed different Rf values among the fractions, indicating differences in alkaloid composition. UV–Visible spectrophotometric analysis showed maximum absorption wavelengths ranging from 284 to 288 nm, which are characteristic of erythrinan alkaloids. These findings confirm that Erythrina subumbrans stem bark contains alkaloid compounds and demonstrate that UAE is an effective extraction method for producing alkaloid-rich fractions suitable for further phytochemical and pharmacological investigations.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Erythrina subumbrans, alkaloid, UAE, fraksinasi & UV-Vis. --- Erythrina subumbrans, alkaloids, UAE, fractionation & UV-Vis |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 19 Sep 2026 02:29 |
| Last Modified: | 19 Sep 2026 02:29 |
| URI: | http://repository.stfi.ac.id/id/eprint/4248 |
Actions (login required)
 |
View Item |
