REGINA VIRA PUTRI SULISTIANDARI, - (2025) OPTIMASI PEREAKSI UNTUK DETEKSI DINI BETA THALASEMIA METODE NAKED EYE SINGLE TUBE RED CELL OSMOTIC FRAGILITY TEST (NESTROFT). Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Thalasemia merupakan kelainan darah herediter yang disebabkan oleh gangguan sintesis rantai globin pada hemoglobin, terutama rantai beta. Deteksi dini terhadap pembawa sifat thalasemia penting untuk mencegah kelahiran anak dengan thalasemia mayor. Penelitian ini bertujuan untuk menentukan konsentrasi optimum reagen BRT (Biolab’s Rapid Thalassemia) dalam metode Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) untuk deteksi dini β-thalassemia. Penelitian dilakukan secara eksperimental menggunakan tiga variasi konsentrasi NaCl yaitu 0,32%, 0,36%, dan 0,40%. Pengujian dilakukan terhadap sampel darah thalasemia mayor, carrier, dan darah normal dengan pengamatan kekeruhan secara visual. Hasil penelitian menunjukkan bahwa variasi konsentrasi NaCl berpengaruh terhadap tingkat kekeruhan larutan. Reagen dengan konsentrasi NaCl 0,36% menghasilkan kekeruhan paling jelas dan konsisten pada sampel thalasemia dibandingkan konsentrasi 0,32% yang mengalami hemolisis berlebih dan 0,40% yang terlalu jernih. Dengan demikian, reagen BRT dengan NaCl 0,36% dinilai paling optimum untuk membedakan sampel darah normal dan sampel thalasemia. Metode NESTROFT dengan reagen BRT terbukti efektif, sederhana, cepat, dan ekonomis, sehingga berpotensi digunakan sebagai skrining awal thalasemia di fasilitas kesehatan dasar. -------- Thalassemia is a hereditary blood disorder caused by impaired synthesis of the globin chains of hemoglobin, particularly the beta chain. Early detection of thalassemia carriers is crucial to prevent the birth of children with severe thalassemia. This study aims to determine the optimum concentration of Biolab’s Rapid Thalassemia (BRT) reagent in the Naked Eye Single Tube Red Cell Osmotic Fragility Test (NESTROFT) method for early detection of Beta thalassemia. The research was conducted experimentally using three variations of NaCl concentrations: 0.32%, 0.36%, and 0.40%. Blood samples from patients with Beta thalassemia major, Beta thalassemia carrier, and normal subjects were tested, and the turbidity of the solution was observed visually. The results showed that different NaCl concentrations affected the degree of turbidity in the solution. The BRT reagent containing 0.36% NaCl produced the most distinct and consistent turbidity in thalassemia samples compared to 0.32% (which caused excessive hemolysis) and 0.40% (which appeared too clear). Therefore, the 0.36% NaCl concentration was found to be the most optimal formulation for distinguishing between thalassemia and normal blood samples. This study concludes that the BRT reagent combined with the NESTROFT method is effective, rapid, simple, and cost-efficient, making it suitable for early screening of thalassemia, particularly in primary healthcare or laboratories with limited facilities.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Thalasemia, deteksi dini, NESTROFT, reagen BRT, NaCl. ------ Thalassemia, early detection, NESTROFT, BRT reagent, NaCl. |
| Subjects: | R Medicine > R Medicine (General) R Medicine > RS Pharmacy and materia medica |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 21 Nov 2025 03:45 |
| Last Modified: | 21 Nov 2025 03:45 |
| URI: | http://repository.stfi.ac.id/id/eprint/3548 |
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