AFIYFAH ISNA NURHAMIDA, - (2025) APLIKASI SOLID PHASE EXTRACTION (SPE) UNTUK PEMURNIAN SENYAWA KURKUMIN MENGGUNAKAN SPEKTROFOTOMETER UV-VISIBEL DAN KCKT. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
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Abstract
Kurkumin merupakan senyawa aktif utama dalam kunyit (Curcuma longa L.) yang memiliki berbagai aktivitas farmakologis, termasuk antiinflamasi, antioksidan, dan antikanker. Penelitian ini bertujuan untuk memurnikan isolat kurkumin 70% menggunakan metode Solid Phase Extraction (SPE) dengan fase diam C18, serta menganalisis tingkat kemurniannya menggunakan Spektrofotometri UV-Vis dan Kromatografi Cair Kinerja Tinggi (KCKT). Proses SPE dilakukan melalui tahap pengkondisian kartrid dengan metanol, pencucian menggunakan metanol 10%, dan elusi dengan metanol murni. Analisis KCKT dilakukan menggunakan kolom XTerra® C18 (4,6 × 150 mm) dengan laju alir 1,0 mL/menit, detektor UV-DAD pada panjang gelombang 425 nm, fase gerak asetonitril : asam asetat 2% dalam air (50:50, v/v), serta volume injeksi 10 μL. Berdasarkan pengukuran menggunakan spektrofotometer UV-Vis dengan persamaan regresi linier y = 0,1421x – 0,0341 dan koefisien korelasi r = 0,9998, diperoleh konsentrasi kurkumin sebelum proses SPE sebesar 9,571 ppm dan setelah proses SPE sebesar 5,357 ppm. Hasil analisis KCKT menunjukkan peningkatan kemurnian isolat dari 87,33% menjadi 100% setelah proses SPE. Dengan demikian, metode SPE terbukti efektif dalam menghilangkan pengotor dan meningkatkan kemurnian isolat kurkumin. ------- Curcumin is the principal active compound in turmeric (Curcuma longa L.) and possesses various pharmacological activities, including anti-inflammatory, antioxidant, and anticancer properties. This study aims to purify a 70% curcumin isolate using the Solid Phase Extraction (SPE) method with a C18 stationary phase, and to evaluate its purity using UV-Visible spectrophotometry and HighPerformance Liquid Chromatography (HPLC). The SPE process consisted of cartridge conditioning with methanol, washing with 10% methanol, and elution with pure methanol. HPLC analysis was performed using an X-Terra® C18 column (4.6 × 150 mm) with a flow rate of 1.0 mL/min, UV-DAD detector at a wavelength of 425 nm, mobile phase of acetonitrile: 2% acetic acid in water (50:50, v/v), and an injection volume of 10 μL. Based on UV-Vis spectrophotometric measurements with a linear regression equation y = 0.1421x – 0.0341 and a correlation coefficient r = 0.9998, curcumin concentrations before and after the SPE process were 9.571 ppm and 5.357 ppm, respectively. HPLC analysis showed an increase in isolate purity from 87.33% to 100% following SPE treatment. Therefore, the SPE method proved to be effective in removing impurities and enhancing the purity of the curcumin isolate.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Kurkumin, Solid Phase Extraction (SPE), Spektrofotometer UV-Vis, KCKT. ------ Curcumin, Solid Phase Extraction (SPE), UV-Vis Spectrophotometry, HPLC. |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 20 Nov 2025 04:47 |
| Last Modified: | 20 Nov 2025 04:47 |
| URI: | http://repository.stfi.ac.id/id/eprint/3533 |
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