PENGARUH KURKUMIN TERHADAP INDUKSI MEKANISME KEMATIAN SEL PADA SEL KANKER PROSTAT DU-145 DENGAN METODE FLOW CYTOMETRY

FRIESKA FITRI YANI, - (2025) PENGARUH KURKUMIN TERHADAP INDUKSI MEKANISME KEMATIAN SEL PADA SEL KANKER PROSTAT DU-145 DENGAN METODE FLOW CYTOMETRY. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.

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Abstract

Kanker prostat merupakan salah satu penyebab utama kematian yang sangat tinggi di populasi pria. Kurkumin senyawa aktif dari rimpang kunyit (Curcuma longa L) memiliki potensi sebagai agen antikanker. Penelitian ini bertujuan untuk menilai efek kurkumin pada aktivasi jalur apoptosis pada sel kanker prostat DU-145 menggunakan analisis flow cytometry. Analisis viabillitas dan apoptosis dilakukan menggunakan pewarnaan Annexin V-FITC dan PI yang dibaca dengan flow cytometry. Sel DU-145 diperbanyak sampai menunjukan hasil yang baik, diikuti oleh paparan kontrol negatif, docetaxel sebagai kontrol positif, dan kurkumin. Evaluasi flow cytometry dilakukan untuk memastikan distribusi sel dalam kuadran yang menunjukkan apoptosis awal, apoptosis lanjut, dan nekrosis. Temuan menunjukkan bahwa pada kelompok kontrol negatif, viabilitas sel tetap meningkat (90,20%) dengan tingkat apoptosis total yang rendah (9,36%). Sebaliknya, kontrol positif docetaxel menunjukkan penurunan viabilitas sel (77,80%) di samping peningkatan yang nyata dalam apoptosis total (21,57%). Sementara itu, kurkumin lebih efektif menghasilkan penurunan viabilitas sel hingga 79,99% dan peningkatan apoptosis total menjadi 16,55%, serta peningkatan nekrosis sebesar 3,63%. Kurkumin memiliki kemampuan untuk menginduksi apoptosis pada sel DU-145, meskipun kemanjurannya lebih rendah daripada docetaxel. Temuan ini menunjukan bahwa kurkumin memiliki potensi sebagai agen kanker alami yang dapat menjadi altenatif dalam terapi kanker prostat. ------- Prostate cancer is one of the leading causes of death in the male population. Curcumin, an active compound from turmeric rhizome (Curcuma longa L.), has potential as an anticancer agent. This study aims to assess the effect of curcumin on the activation of the apoptosis pathway in DU-145 prostate cancer cells using flow cytometry analysis. Viability and apoptosis analysis were performed using Annexin V-FITC and PI staining, read by flow cytometry. DU-145 cells were propagated until they showed good results, followed by exposure to negative controls, docetaxel as a positive control, and curcumin. Flow cytometry evaluation was performed to ensure cell distribution in quadrants indicating early apoptosis, late apoptosis, and necrosis. The findings showed that in the negative control group, cell viability remained increased (90.20%) with a low level of total apoptosis (9.36%). In contrast, the positive control docetaxel showed a decrease in cell viability (77.80%) in addition to a marked increase in total apoptosis (21.57%). Meanwhile, curcumin was more effective, reducing cell viability by 79.99% and increasing total apoptosis by 16.55%, as well as increasing necrosis by 3.63%. Curcumin was able to induce apoptosis in DU-145 cells, although its efficacy was lower than docetaxel. These findings suggest that curcumin has potential as a natural anticancer agent that could be an alternative in prostate cancer therapy.

Item Type:	Thesis (Skripsi)
Uncontrolled Keywords:	Kurkumin, DU-145, kanker prostat, apoptosis, flow cytometry. ------- Curcumin, DU-145, prostate cancer, apoptosis, flow cytometry
Subjects:	R Medicine > R Medicine (General) R Medicine > RM Therapeutics. Pharmacology
Divisions:	Program Studi S1 Farmasi
Depositing User:	pustakawan - -
Date Deposited:	20 Nov 2025 02:42
Last Modified:	20 Nov 2025 02:42
URI:	http://repository.stfi.ac.id/id/eprint/3516

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