DINA ALIFA MAULUDIYAH, - (2025) APLIKASI SOLID PHASE EXTRACTION (SPE) UNTUK PEMURNIAN SENYAWAALFA MANGOSTIN DARI ISOLAT ALFA MANGOSTIN. Skripsi thesis, Sekolah Tinggi Farmasi Indonesia.
![[img]](http://repository.stfi.ac.id/style/images/fileicons/text.png) |
Text
S_PSSF_A242008_Title.pdf Download (601kB) |
|
Text
S_PSSF_A242008_Chapter1.pdf Restricted to Repository staff only Download (237kB) |
|
Text
S_PSSF_A242008_Chapter2.pdf Restricted to Repository staff only Download (450kB) |
|
Text
S_PSSF_A242008_Chapter3.pdf Restricted to Repository staff only Download (264kB) |
|
Text
S_PSSF_A242008_Chapter4.pdf Restricted to Repository staff only Download (465kB) |
|
Text
S_PSSF_A242008_Chapter5.pdf Restricted to Repository staff only Download (244kB) |
|
Text
S_PSSF_A242008_Appendix.pdf Restricted to Repository staff only Download (816kB) |
Abstract
Kulit buah manggis (Garcinia mangostana L.) mengandung senyawa bioaktif utama, yaitu alfa mangostin yang memiliki aktivitas farmakologis sebagai antiinflamasi, antihiperglikemia, antidiabetes, dan antikanker. Isolat alfa mangostin dalam penelitian ini memiliki tingkat kemurnian awal sebesar 83,34% berdasarkan analisis Kromatografi Cair Kinerja Tinggi (KCKT). Namun, hasil isolat tersebut masih mengandung senyawa lain sehingga diperlukan proses pemurnian lanjutan untuk memperoleh kemurnian yang lebih tinggi. Penelitian ini bertujuan untuk menentukan efektivitas kartrid C-18 dalam meningkatkan kemurnian isolat alfa mangostin menggunakan metode Solid Phase Extraction (SPE). Identifikasi senyawa alfa mangostin dilakukan secara kualitatif menggunakan Kromatografi Lapis Tipis (KLT), sedangkan analisis kuantitatif menggunakan Spektrofotometer UV-Visibel dan Kromatografi Cair Kinerja Tinggi (KCKT). Hasil analisis Spektrofotometer UV-Vis menunjukkan penurunan konsentrasi setelah proses SPE, dari 7,248 ppm menjadi 1,720 ppm yang menandakan berkurangnya pengotor dalam sampel. Sementara itu, hasil KCKT menunjukkan peningkatan kemurnian isolat secara signifikan dengan % area meningkat dari 73,13% menjadi 100% dengan waktu retensi sekitar 9,4-9,6 menit. Dengan demikian, metode Solid Phase Extraction (SPE) berbasis kartrid C-18 terbukti efektif, selektif, dan ekonomis dalam meningkatkan kemurnian isolat alfa mangostin. ------- Mangosteen peel (Garcinia mangostana L.) contains a major bioactive compound, alpha mangostin, which has pharmacological activities as an anti-inflammatory, antihyperglycemic, antidiabetic, and anticancer agent. The alpha mangostin isolate in this study had an initial purity level of 83.34% based on High Performance Liquid Chromatography (HPLC) analysis. However, the isolate still contained other compounds, requiring further purification to obtain higher purity. This study aimed to determine the effectiveness of the C-18 cartridge in increasing the purity of alpha mangostin isolate using the Solid Phase Extraction (SPE) method. The identification of alpha mangostin compounds was carried out qualitatively using Thin Layer Chromatography (TLC), while quantitative analysis was carried out using a UV-Visible Spectrophotometer and High Performance Liquid Chromatography (HPLC). The results of the UV-Vis Spectrophotometer analysis showed a decrease in concentration after the SPE process, from 7,248 ppm to 1,720 ppm, indicating a reduction in impurities in the sample. Meanwhile, the HPLC results showed a significant increase in isolate purity with an area percentage increasing from 73.13% to 100% with a retention time of approximately 9.4-9.6 minutes. Thus, the C-18 cartridge-based Solid Phase Extraction (SPE) method proved to be effective, selective, and economical in increasing the purity of alpha mangostin isolates.
| Item Type: | Thesis (Skripsi) |
|---|---|
| Uncontrolled Keywords: | Solid Phase Extraction (SPE), alfa mangostin, Kromatografi Lapis Tipis (KLT), Spektrofotometri UV-Visible, dan KCKT. ------ Solid Phase Extraction (SPE), alpha-mangostin, Thin Layer Chromatography (TLC), UV-Visible spectrophotometry, and HPLC. |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry |
| Divisions: | Program Studi S1 Farmasi |
| Depositing User: | pustakawan - - |
| Date Deposited: | 20 Nov 2025 02:13 |
| Last Modified: | 20 Nov 2025 02:13 |
| URI: | http://repository.stfi.ac.id/id/eprint/3512 |
Actions (login required)
 |
View Item |
